عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304049726
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Mycobacterium bovis infection in cattle in Morocco:
نام عام مواد
[Thesis]
نام نخستين پديدآور
J. Berrada
عنوان اصلي به قلم نويسنده ديگر
Preparation and evaluation of chemical extracts for use in detection of immune responses
نام ساير پديدآوران
C. O. Thoen
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Iowa State University
تاریخ نشرو بخش و غیره
1993
مشخصات ظاهری
نام خاص و کميت اثر
201
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
Iowa State University
امتياز متن
1993
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Sodium deoxycholate (DOC) was utilized to extract immunologically-active cell wall components of virulent M. bovis and M. avium, isolated from cattle in Morocco and M. fortuitum as well as related organisms e.g., N. asteroides and R. equi. The extracts from M. bovis, N. asteroides, M. fortuitum, and M. avium, were fractionated by size exclusion chromatography using Sephadex S-200. Two fractions (1 and 2) were obtained at absorbance of 280 nm. Fraction 1 of M. bovis, M. avium, M. fortuitum and of N. asteroides and nonfractionated DOC extract of R. equi elicited delayed-type hypersensitivity responses in homologously or heterologously sensitized guinea-pigs. Significant enzyme-linked immunosorbent assay values were obtained using sera from cattle 4 weeks after sensitization with homologous bacterial preparations (p < 0.01). Significant ELISA cross-reactions were observed in M. bovis or M. avium sensitized calves, using heterologous antigens. Mycobacteriologic examinations conducted on tissues collected from 246 cows in 5 major abattoirs of Morocco, revealed that M. bovis was isolated from 29.67% of the cattle examined. Mycobacteria other than M. bovis (MOTT) were isolated from tissues of 23 carcasses (9.34%). Three had lesions compatible with tuberculosis; M. bovis was not isolated. An indirect enzyme-linked immunosorbent assay using M. bovis PPD or M. bovis DOC fraction 1 as antigens was used for detecting antibodies in sera of cattle from which M. bovis was isolated. The sensitivity for M. bovis PPD was 76.71% and for DOC extract of M. bovis was 79.45%; the specificity was of 90% and 92%, respectively. These values are similar to those achieved by the tuberculin skin test (71). Investigations conducted on five selected herds in Morocco using the cervical intradermal tuberculin test and ELISA revealed that cattle in three herds were tuberculous. The comparative cervical tuberculin test using M. bovis and M. avium PPD's at equal concentration was conducted within 24 hours of a positive or suspect skin response; no reactors were identified in the remaining two herds. Combination of ELISA and tuberculin skin test conducted on animals from tuberculous herds was found to be more sensitive than either test conducted alone.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
immune response
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Veterinary services
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
C. O. Thoen
مستند نام اشخاص تاييد نشده
J. Berrada
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
