عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq303815454
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Characterization of an iron-regulated outer membrane protein of Pasteurella multocida
نام عام مواد
[Thesis]
نام نخستين پديدآور
J. S. Ikeda
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of California, Davis
تاریخ نشرو بخش و غیره
1990
مشخصات ظاهری
نام خاص و کميت اثر
302
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of California, Davis
امتياز متن
1990
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Pasteurella multocida strain P1059 (serotype 3) expressed an 84 kilodalton (kDa) outer membrane protein (OMP) when grown in brain heart infusion (BHI) broth containing the iron chelator dipyridyl (20 mug/ml) but not in BHI alone. The OMP was also expressed by P. multocida strain P1059 when grown in turkey plasma or BHI containing Desferal (20.5 mug/ml). The proteinaceous nature of the OMP was confirmed and the OMP was demonstrated to be at the surface of the bacterial cell. Plasma collected from turkeys naturally infected with P. multocida (serotype 3) contained antibodies to the OMP. Polyclonal and monoclonal antibodies specific to the 84 kDa OMP were prepared. It was shown that antigenically related iron-regulated OMPs were expressed by other serotypes of P. multocida. Turkeys passively immunized with the polyclonal antibodies had a greater mean death time and percent survival compared to unimmunized turkeys following challenge with P. multocida. The gene (or part of the gene) was cloned and sequenced in order to determine a function of the protein by comparison with previously sequenced procaryotic genes. A 2033 bp EcoRI DNA fragment was cloned into lambda gt11 which expressed a fusion product that reacted with the polyclonal antibodies specific to the 84 kDa OMP. It was believed that a portion of the gene was cloned corresponding to the carboxy terminal end of the protein. The DNA had some homologies with the Escherichia coli ferric hydroxamate uptake operon suggesting a role in iron acquisition. It was concluded that the 84 kDa OMP might be a suitable candidate for inclusion in an immunizing agent against fowl cholera.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
fowl cholera
موضوع مستند نشده
Microbiology
موضوع مستند نشده
Molecular biology
موضوع مستند نشده
outer membrane protein
موضوع مستند نشده
Veterinary services
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
J. S. Ikeda
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
