عنوان

Transfer, expression and inheritance of growth hormone genes in channel catfish (Ictalurus punctatus) and common carp (Cyprinus carpio)

شماره

TLpq303723981

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Transfer, expression and inheritance of growth hormone genes in channel catfish (Ictalurus punctatus) and common carp (Cyprinus carpio)

نام عام مواد

[Thesis]

نام نخستين پديدآور

M. Hayat

نام ساير پديدآوران

R. A. Dunham

نام ناشر، پخش کننده و غيره

Auburn University

تاریخ نشرو بخش و غیره

1989

نام خاص و کميت اثر

139

جزئيات پايان نامه و نوع درجه آن

Ph.D.

کسي که مدرک را اعطا کرده

Auburn University

امتياز متن

1989

متن يادداشت

Channel catfish and common carp embryos were microinjected with mouse metallothionein human growth hormone fusion gene (MThGH), two constructs of RSV rainbow trout growth hormone cDNA (pRSVrtGHcDNA), RSV coho salmon growth hormone cDNA (pRSVcsGHcDNA) and rainbow trout vitellogenin gene (rtVG20) at the early, intermediate and late one-cell stage and at the two-cell and four-cell stages to determine the developmental stage appropriate for maximizing production of transgenic fish. Survival (hatching rate) of microinjected channel catfish embryos relative to non-injected controls was 65.0, 43.6, 38.5, 27.4 and 35.9% for early, intermediate and late one-cell, two-cell and four-cell stages, respectively. Survival of microinjected common carp embryos relative to non-injected controls was 88.9, 85.3, 91.5, 85.3 and 83.4% for early, intermediate and late one-cell, two-cell and four-cell stages, respectively. Twenty-two of 420 (5.2%) microinjected channel catfish analyzed contained sequences of foreign DNA, and the frequency of integration was 2.1, 1.5, 15.2, 12.5 and 7.7% at the early, intermediate and late one-cell, two-cell and four-cell stages. Twenty-five of 533 (4.7%) microinjected common carp contained sequences of foreign DNA and the frequency of integration was 8.3, 1.0, 0.0, 11.5 and 1.7% at the five respective developmental stages. All transgenic common carp (pRSVrtGHcDNA-construct 1) produced trout GH polypeptide. The quantity of growth hormone detected ranged from 8.0 ng/mg to 89.1 ng/mg of protein in red blood cells. Ninety-day-old transgenic common carp that developed from microinjection of pRSVrtGHcDNA at early one-cell stage grew 20% faster (P < 0.05) than normal siblings. Those transgenic individuals microinjected at the two-cell stage grew at the same rate as normal siblings. Three of 4 male transgenic common carp tested transmitted the foreign DNA to their progeny. Positive F progeny grew 20-40% faster (P < 0.001) than their normal siblings, and 32-46% of these individuals were larger than the largest control at 90 days of age.

موضوع مستند نشده

Aquaculture

موضوع مستند نشده

Biological sciences

موضوع مستند نشده

Catfish

موضوع مستند نشده

Fish production

موضوع مستند نشده

Forestry

موضوع مستند نشده

Genetics

مستند نام اشخاص تاييد نشده

M. Hayat

مستند نام اشخاص تاييد نشده

R. A. Dunham

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y