A Study of Bacterial Translation at Codon Resolution Using Ribosome Profiling
نام عام مواد
[Thesis]
نام نخستين پديدآور
Mohammad, Fuad
نام ساير پديدآوران
Nathans, Jeremy
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
The Johns Hopkins University
تاریخ نشرو بخش و غیره
2020
مشخصات ظاهری
نام خاص و کميت اثر
178
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
The Johns Hopkins University
امتياز متن
2020
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Ribosome profiling has pushed the boundary of how translation is studied by illuminating every step of the translation cycle at the genome scale. First developed by Nick Ingolia and Jonathan Weismann, ribosome profiling is now widely used in both bacterial and eukaryotic studies. However, development of the method in bacteria has not achieved the level of refinement seen in yeast and mammalian ribosome profiling. This thesis focuses on analyzing the current methodology in bacteria to understand its strengths and shortcomings and developing improvements in both how libraries are prepared and how the data is analyzed. This thesis will also focus on implementing these improvements to understand events that influence translation elongation as well as how ribosome profiling can be used to identify new genes.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Bioinformatics
موضوع مستند نشده
Molecular biology
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )