Modulation of energy homeostasis in maize and Arabidopsis to develop lines tolerant to drought, genotoxic and oxidative stresses
نام عام مواد
[Article]
نام نخستين پديدآور
Elizabeth Njuguna, Griet Coussens, Stijn Aesaert, et al.
وضعیت نشر و پخش و غیره
محل نشرو پخش و غیره
Leiden
نام ناشر، پخش کننده و غيره
Brill
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Abiotic stresses cause crop losses worldwide that reduce the average yield by more than 50%. Due to the high energy consumed to enhance the respiration rates, the excessive reactive oxygen species release provokes cell death and, ultimately, whole plant decay. A metabolic engineering approach in maize (Zea mays) altered the expression of two poly(ADP-ribosyl)ation metabolic pathway proteins, poly(ADP-ribose) polymerase (PARP) and ADP-ribose-specific Nudix hydrolase (NUDX) genes that play a role in the maintenance of the energy homeostasis during stresses. By means of RNAi hairpin silencing and CRJSPR/Cas9 gene editing strategies, the PARP expression in maize was downregulated or knocked down. The Arabidopsis NUDX7 gene and its two maize homologs, ZmNUDX2 and ZmNUDX8, were overexpressed in maize and Arabidopsis. Novel phenotypes were observed, such as significant tolerance to oxidative stress and improved yield in Arabidopsis and a trend of tolerance to mild drought stress in maize and in Arabidopsis. Abiotic stresses cause crop losses worldwide that reduce the average yield by more than 50%. Due to the high energy consumed to enhance the respiration rates, the excessive reactive oxygen species release provokes cell death and, ultimately, whole plant decay. A metabolic engineering approach in maize (Zea mays) altered the expression of two poly(ADP-ribosyl)ation metabolic pathway proteins, poly(ADP-ribose) polymerase (PARP) and ADP-ribose-specific Nudix hydrolase (NUDX) genes that play a role in the maintenance of the energy homeostasis during stresses. By means of RNAi hairpin silencing and CRJSPR/Cas9 gene editing strategies, the PARP expression in maize was downregulated or knocked down. The Arabidopsis NUDX7 gene and its two maize homologs, ZmNUDX2 and ZmNUDX8, were overexpressed in maize and Arabidopsis. Novel phenotypes were observed, such as significant tolerance to oxidative stress and improved yield in Arabidopsis and a trend of tolerance to mild drought stress in maize and in Arabidopsis.
مجموعه
تاريخ نشر
2017
توصيف ظاهري
66-76
عنوان
Afrika Focus
شماره جلد
30/2
شماره استاندارد بين المللي پياييندها
2031-356X
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
CRISPR/Cas9
اصطلاح موضوعی
drought stress
اصطلاح موضوعی
maize
اصطلاح موضوعی
Nudix hydrolase
اصطلاح موضوعی
oxidative stress
اصطلاح موضوعی
poly(ADP-ribose) polymerase
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )