عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL52985
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Análisis de casos de artritis asociada a reovirus en pavos y desarrollo de una prueba serológica
نام عام مواد
[Thesis]
نام نخستين پديدآور
Barrera Vaca, Maria Soledad
نام ساير پديدآوران
Goyal, Sagar M.
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Minnesota
تاریخ نشرو بخش و غیره
2019
يادداشت کلی
متن يادداشت
55 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.S.
کسي که مدرک را اعطا کرده
University of Minnesota
امتياز متن
2019
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Turkey reovirus (TRV) has been associated with several clinical syndromes including lameness, enteritis, myocarditis, and immunosuppression in turkey flocks. Lameness and enteritis are the current field problems and an increase in cases of lameness has been reported, possibly due to the emergence of novel genotypes of the virus. It is, therefore, important to understand factors responsible for this increase and to devise methods for the rapid detection of the virus and its antibodies. Currently, TRV is detected through RT-PCR and virus isolation but no specific serological test is available to detect anti-TRV antibodies in turkeys. In this thesis, I report a retrospective analysis of lameness submissions to the University of Minnesota Veterinary Diagnostic Laboratory (UMN-VDL) in the last eight years and confirm that submission of TRV positive cases has indeed increased. There were some months during which the probability of positive cases was numerically high. In addition, the submission of reovirus-positive lameness cases came from many different states indicating that the problem is widespread. There was a positive correlation between age of the turkey and TRV-related lameness; the number of TRV positive cases increased as the flocks aged. In addition, I developed a whole-virus ELISA (enzyme-linked immunosorbent assay) to detect anti-TRV antibodies. The TRV isolate used for this ELISA was a representative of several TRV genotypes based on Sigma C and B conserved epitopes. The test was validated using serum samples from experimental and clinical cases of TRV. At 0.4 cut-off, the diagnostic sensitivity and specificity of this ELISA were 0.92. In summary, the development of an ELISA with high sensitivity and specificity should provide an opportunity for early diagnosis of reovirus exposure and to follow the dynamics of reovirus immune response over time.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Animal diseases
اصطلاح موضوعی
Veterinary medicine
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Barrera Vaca, Maria Soledad
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Goyal, Sagar M.
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
University of Minnesota
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
