عنوان

Immunochemical protocols /

شابک

0896032043

شابک

0896032701

شابک

1592594972

شابک

9780896032040

شابک

9780896032705

شابک

9781592594979

شماره

b777727

عنوان اصلي

Immunochemical protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Margaret M. Manson.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©1992.

نام خاص و کميت اثر

xiii, 480 pages :

ساير جزييات

illustrations ;

ابعاد

24 cm

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 10

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Production of polyclonal antisera -- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens -- Raising polyclonal antibodies using nitrocellulose-bound antigen -- Synthesis of peptides for use as immunogens -- Production and characterization of antibodies against synthetic peptides -- Preparation and testing of monoclonal antibodies to recombinant proteins -- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase -- Purification of immunoglobulin G (IgG) -- Epitope mapping -- Enzyme-antienzyme method for immunohistochemistry -- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates -- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies -- Avidin-biotin technology: preparation of biotinylated probes -- Avidin-biotin technology: preparation of avidin conjugates -- Immunochemical applications of avidin-biotin technology -- Preparation of gold probes -- Immunogold probes for light microscopy -- Immunogold probes in electron microscopy -- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species -- Quantitative and qualitative immunoelectrophoresis -- Rocket immunoelectrophoresis techniques or electroimmunodiffusion -- Crossed immunoelectrophoresis -- Crossed immunoaffinoelectrophoresis -- Immunodetection of proteins by western blotting -- Erasable western blots -- Colloidal gold staining and immunoprobing on the same western blot -- Colloidal gold staining and immunodetection in 2-D protein mapping -- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen -- Competitive ELISA -- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system -- Preparation of cytotoxic antibody-toxin conjugates -- Immunoaffinity purification and quantification of antibody-toxin conjugates -- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA -- Production of monoclonal antibodies for the detection of chemically modified DNA -- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA -- An immunochemical assay for detecting transition of B-DNA to Z-DNA -- Cell sorting using immunomagnetic beads -- Cell preparation for flow cytometry -- Preparation of rat lung cells for flow cytometry -- The isolation of rat hepatocytes for flow cytometry -- Flow cytometric analysis of cells using an antibody to a surface antigen -- Multiple immunofluorescence analysis of cells using flow cytometry -- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine -- Production and use on nonradioactive hybridization probes -- Cellular human and viral DNA detection by nonisotopic in situ hybridization -- Chromosomal mapping of genes by nonisotopic in situ hybridization -- Nonisotopic in situ hybridization -- Biotinylated probes in colony hybridization -- Screening of agt11 cDNA libraries using monoclonal antibodies -- Expression of foreign genes in mammalian cells using an antibody fusion system.

بدون عنوان

0

عنوان

Immunochemical protocols.

موضوع مستند نشده

Immunochemistry-- Methodology.

موضوع مستند نشده

Antibodies-- isolation & purification.

موضوع مستند نشده

Immunochemistry-- methods.

موضوع مستند نشده

Immunochimie-- Méthodologie.

موضوع مستند نشده

Aufsatzsammlung

موضوع مستند نشده

Immunchemie

موضوع مستند نشده

Immunochemie.

موضوع مستند نشده

Immunochemistry-- Methodology.

موضوع مستند نشده

Immunochimie-- Technique.

موضوع مستند نشده

Immunochimie.

موضوع مستند نشده

Methode

موضوع مستند نشده

Techniques immunologiques.

موضوع مستند نشده

Wetenschappelijke technieken.

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

شماره رده

نشانه اثر

نشانه اثر

کد سيستم

کد سيستم

کد سيستم

کد سيستم

کد سيستم

کد سيستم

مستند نام اشخاص تاييد نشده

Manson, Margaret M.

تاريخ عمليات

20201203110916.0

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y