عنوان

Directed evolution library creation :

شابک

1280843233

شابک

1588292851

شابک

159259395X

شابک

6610843236

شابک

9781280843235

شابک

9781588292858

شابک

9781592593958

شابک

9786610843237

شابک اشتباه

9781617374715

شماره

b768765

عنوان اصلي

Directed evolution library creation :

نام عام مواد

[Book]

ساير اطلاعات عنواني

methods and protocols /

نام نخستين پديدآور

edited by Frances H. Arnold and George Georgiou.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2003.

نام خاص و کميت اثر

1 online resource (x, 224 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 231

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Cover -- Preface -- Contents -- Contributors -- MUTAGENESIS AND RECOMBINATION METHODS -- 1 Generating Mutant Libraries Using Error-Prone PCR -- 2 Preparing Libraries in Escherichia coli -- 3 Preparing Libraries in Saccharomyces cerevisiae -- 4 Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP) -- 5 Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis -- 6 Production of Randomly Mutated Plasmid Libraries Using Mutator Strains -- 7 Evolution of Microorganisms Using Mutator Plasmids -- 8 Random Insertion and Deletion Mutagenesis -- 9 Random Oligonucleotide Mutagenesis -- 10 Saturation Mutagenesis -- 11 DNA Shuffling -- 12 Family Shuffling with Single-Stranded DNA -- 13 In Vitro DNA Recombination by Random Priming -- 14 Staggered Extension Process (StEP) In Vitro Recombination -- 15 RACHITT -- 16 The Creation of ITCHY Hybrid Protein Libraries -- 17 Preparation of SCRATCHY Hybrid Protein Libraries -- 18 Sequence Homology-Independent Protein Recombination (SHIPREC) -- 19 Producing Chimeric Genes by CLERY -- ANALYSIS OF LIBRARY DIVERSITY -- 20 Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization -- 21 Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays -- 22 Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays -- Index -- Last Page.

بدون عنوان

0

متن يادداشت

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

عنوان

Directed evolution library creation.

شماره استاندارد بين المللي کتاب و موسيقي

9781588292858

موضوع مستند نشده

Combinatorial chemistry, Laboratory manuals.

موضوع مستند نشده

Gene libraries, Laboratory manuals.

موضوع مستند نشده

Genetic engineering, Laboratory manuals.

موضوع مستند نشده

Genetic recombination, Laboratory manuals.

موضوع مستند نشده

Microbial mutation, Laboratory manuals.

موضوع مستند نشده

Molecular evolution, Laboratory manuals.

موضوع مستند نشده

Combinatorial Chemistry Techniques.

موضوع مستند نشده

Gene Library.

موضوع مستند نشده

Banques de gènes, Manuels de laboratoire.

موضوع مستند نشده

Chimie combinatoire, Manuels de laboratoire.

موضوع مستند نشده

Évolution moléculaire, Manuels de laboratoire.

موضوع مستند نشده

Génie génétique, Manuels de laboratoire.

موضوع مستند نشده

Micro-organismes-- Mutation, Manuels de laboratoire.

موضوع مستند نشده

Recombinaison génétique, Manuels de laboratoire.

موضوع مستند نشده

Biologia molecular.

موضوع مستند نشده

Combinatorial chemistry.

موضوع مستند نشده

Engenharia genética.

موضوع مستند نشده

Gene libraries.

موضوع مستند نشده

Genetic engineering.

موضوع مستند نشده

Genetic recombination.

موضوع مستند نشده

Genetica.

موضوع مستند نشده

Genetische manipulatie.

موضوع مستند نشده

Microbial mutation.

موضوع مستند نشده

Moleculaire biologie.

موضوع مستند نشده

Molecular evolution.

موضوع مستند نشده

Mutagênese.

موضوع مستند نشده

Recombinação genética.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Molecular Biology.

موضوع مستند نشده

Wetenschappelijke technieken.

موضوع مستند نشده

PSF.

موضوع مستند نشده

SCI-- 049000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

Arnold, Frances Hamilton.

مستند نام اشخاص تاييد نشده

Georgiou, George.

تاريخ عمليات

20201206143132.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y