عنوان

MAP kinase signaling protocols /

شابک

0896039986

شابک

128035836X

شابک

1592596711

شابک

6610358362

شابک

9780896039988

شابک

9781280358364

شابک

9781592596713

شابک

9786610358366

شماره

b708310

عنوان اصلي

MAP kinase signaling protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Rony Seger.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2004.

نام خاص و کميت اثر

1 online resource (xi, 332 pages) :

ساير جزييات

illustrations

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 250

متن يادداشت

Includes index.

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

The erk cascade as a prototype of mapk signaling pathways / H. Rubinfeld and R. Seger -- Determination of erk activity: Antiphospho-erk antibodies, in vitro phosphorylation, and in-gel kinase assay / S. Kraus and R. Seger -- Detection of erk1/2 activities using affinity reagents / R. Pulido, A. Zuniga and A. Ullrich -- Activation of sapks/jnks and p38s in vitro / J.M. Kyriakis, H. Liu and D.N. Chadee -- Investigating the cellular bmk1/erk5 signaling pathway / R.I. Tapping [and others] -- Pull-down assays for guanoside 5'-triphosphate-bound ras-like guanosine 5'-triphosphatases / M. van Triest and J.L. Bos -- Regulation of mapk cascades by protein tyrosine phosphatases / J. Torres, C. Blanco-Aparicio and R. Pulido -- Use of inhibitors in the study of mapk signaling / Y.D. Shaul and R. Seger -- Structure of mapks / E.J. Goldsmith, M.H. Cobb and C.I. Chang -- Subcellular localization of mapks / M. Adachi and E. Nishida -- Study of mapk signaling using knockout mice / G. Pages and J. Pouyssegur -- Computer simulation of mapk signal transduction / B.D. Aguda and H.M. Sauro -- Signaling by growth factor receptors / B.S. Kochupurakkal and Y. Yarden -- Activation of mapks by g protein-coupled receptors / P. Crespo and J.S. Gutkind -- Identification of mapk substrates by expression screening with solid-phase phosphorylation / R. Fukunaga and T. Hunter -- Study of substrate specificity of mapks using oriented peptide libraries / M.B. Yaffe -- Ras signaling pathway for analysis of protein-protein interactions in yeast and mammalian cells / A. Aronheim -- Methods in functional proteomics: Two-dimensional polyacrylamide gel electrophoresis with immobilized ph gradients, in-gel digestion and identification of proteins by mass spectrometry / K.R. Bernard [and others] -- Practical methods for deuterium exchange/mass spectrometry / A.N. Hoofnagle, K.A. Resing and N.G. Ahn -- Use of xenopus oocytes and early embryos to study mapk signaling / E. Perdiguero and A.R. Nebreda -- Mapk cascades in the brain: Lessons from learning / D.E. Berman and Y. Dudai.

بدون عنوان

0

متن يادداشت

Mitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

شماره انبار

springerp

عنوان

MAP kinase signaling protocols.

شماره استاندارد بين المللي کتاب و موسيقي

0896039986

موضوع مستند نشده

Cellular signal transduction-- Research-- Methodology.

موضوع مستند نشده

Mitogen-activated protein kinases-- Research-- Methodology.

موضوع مستند نشده

MAP Kinase Signaling System

موضوع مستند نشده

Signal Transduction-- physiology

موضوع مستند نشده

Cellular signal transduction-- Research-- Methodology.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Biochemistry.

موضوع مستند نشده

PSB.

موضوع مستند نشده

SCI-- 007000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

Seger, Rony.

تاريخ عمليات

20201207231700.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y