عنوان

Comparison of micro RNA (miRNA) Profiles and Some miRNA Target Gene Expression levels in Roots of Non-infected and Huanglongbing-infected Tangerine (Citrus reticulata Blanco cv.'Sanhu' ) Trees

شماره

LA9k8846mf

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Comparison of micro RNA (miRNA) Profiles and Some miRNA Target Gene Expression levels in Roots of Non-infected and Huanglongbing-infected Tangerine (Citrus reticulata Blanco cv.'Sanhu' ) Trees

نام عام مواد

[Article]

نام نخستين پديدآور

Zhong, Yun; Cheng, Chunzhen; Wu, Bo; Bei, Xuejun; Jiang, Bo; Zeng, Jiwu; Zhong, Guangyan

متن يادداشت

Solexa sequencing was used to reveal the changes in small RNAome profile in roots of mock-inoculated (CK) and Huanglongbing bacteria-inoculated (HLB) 'Sanhu' tangerine (Citrus reticulata Blanco) trees. Results showed that the number of reads of both unique and total sRNAs decreased apparently in roots following infection with HLB. Distribution in length of sRNAs changed also remarkably, showing an increase in 22 nt and 21 nt small RNAs (sRNAs) and a decrease in 24 nt sRNAs in HLB-infected samples. A total of 42 known micro RNAs (miRNAs) belonging to 27 highly conserved miRNA families were identified. Comparisons showed that 33 known miRNAs exhibited a significant expression difference between CK and HLB-inoculated samples. In addition, 34 novel miRNAs, among which 24 were differentially expressed, were also identified, and their expression levels were analyzed by qRT-PCR. Three hundred and eighty five potential target genes were predicted for most of the 57 differentially expressed miRNAs. GO and KEGG annotation analysis revealed that most miRNA-target genes were those implicated in developmental process, response to stress and stimulus, transcription and protein metabolism. The characterization of the miRNAomes between the healthy and HLB infected Sanhu tangerine roots provided new insight into the involvement of miRNAs in HLB infection of citrus.

تاريخ نشر

2014

عنوان

Journal of Citrus Pathology

شماره جلد

1

عنصر شناسه اي

Zhong, Yun; Cheng, Chunzhen; Wu, Bo; Bei, Xuejun; Jiang, Bo; Zeng, Jiwu; Zhong, Guangyan

نام الکترونيکي

نوع ماده

[Article]

کد کاربرگه

275578

سطح دسترسي

a

تكميل شده

Y