عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
LA7sq3t7mr
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Evaluation of Thermotherapy against Huanglongbing (Citrus Greening) under Laboratory Conditions
نام عام مواد
[Article]
نام نخستين پديدآور
Fan, Guocheng; Xia, Yulu; Lin, Xiongjie; Cai, Zijian; Hu, Hanqing; Wang, Xianda; Ruan, Chuanqing; Lu, Lianming; Sequeira, Ronald; Liu, Bo
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Huanglongbing (HLB, citrus greening) is the most destructive disease of citrus. The disease is associated with "Candidatus Liberibacter asiaticus". Few management options are available, besides preventive measures such as the removal of affected plants, planting disease-free stock and maintaining vector-free production in quarantine areas. In this study, we assessed the efficacy of thermotherapy against the disease under controled laboratory conditions. A total of sixty, 2-year old graft-infected Citrus reticulate Blanco seedlings were used for the study. The plants were randomly divided into 3 treatment groups (45, 48°C, and untreated), with 5 plants/rep, 4 reps/trt. The treated plants were placed in temperature chambers for a 4-h treatment sessions, repeated weekly 3 times. Disease remission was observed beginning 8 weeks post-treatment. Real-time PCR assays revealed that pathogen "Ca. L. asiaticus" concentration of all HLB-affected seedlings were significantly reduced except for 8 plants under 45 and 48°C treatments at 4 weeks after treatment. In contrast, pathogen concentration in the untreated control plants exhibited a significant increase, with the highest increase of about 30-fold compared to the initial pathogen concentration (pre-treatment). Except for 7 plants (7 out of 40 total plants), pathogen concentration in the new flushes of the treated plants decreased 90% at ca. 8 weeks after treatment, compared to the initial pathogen concentration. Nested and real-time PCR were used for confirmation of HLB infection in the seedlings, and for pathogen titer assessment.
مجموعه
تاريخ نشر
2014
عنوان
Journal of Citrus Pathology
شماره جلد
1
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
عنصر شناسه اي
Fan, Guocheng; Xia, Yulu; Lin, Xiongjie; Cai, Zijian; Hu, Hanqing; Wang, Xianda; Ruan, Chuanqing; Lu, Lianming; Sequeira, Ronald; Liu, Bo
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب اطلاعات رکورد کتابشناسی
نوع ماده
[Article]
کد کاربرگه
275578
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
