عنوان

Radiohalogenation of small biomolecules

شماره

TLets756431

عنوان اصلي

Radiohalogenation of small biomolecules

نام عام مواد

[Thesis]

نام نخستين پديدآور

Hadi, Usama A. M.

نام ناشر، پخش کننده و غيره

Loughborough University of Technology

تاریخ نشرو بخش و غیره

1978

جزئيات پايان نامه و نوع درجه آن

Thesis (Ph.D.)

امتياز متن

1978

متن يادداشت

Halogenation of tyrosine, uracil, cytosine, uridine and histidine has been attempted-using both the enzymatic catalysis and chemical oxidation labelling methods-with I-125 and Br-80m. Chemical and enzymatic halogenation gave high yields of iodinated tyrosine, uracil, cytosine, uridine, and histidine, and brominated tyrosine, uracil, and cytosine. Reverse-phase high-pressure liquid chromatography separation and gel filtration were both investigated for use in the isolation and analysis of the labelled products. HPLC was most successful and had the advantages that: (1) the technique was fast enough to use with short-lived radiohalogens, i.e. t1⁄2 ≈ 20mins (such as I-128, t1⁄2 = 25 mins); (2) the products were pure and free from interfering impurities. It was found that the use of chemical oxidation and enzymatic catalysis labelling coupled with reverse phase HPLC separation provided a very rapid method of obtaining high specific activity of radiohalogenated compounds in aqueous solution completely free from buffering agents and non-halogenated parent compounds.

مستند نام اشخاص تاييد نشده

Hadi, Usama A. M.

مستند نام تنالگان تاييد نشده

Loughborough University

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y