عنوان

The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations /

شابک

0128011858

شابک

9780128011850

شماره

dltt

عنوان اصلي

The use of CRISPR/Cas9, ZFNs, and TALENs in generating site-specific genome alterations /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Jennifer A. Doudna, Erik J. Sontheimer

وضعيت ويراست

First edition

نام خاص و کميت اثر

xx, 549 pages, 24 unnumbered pages of plates :

ساير جزييات

illustrations (some color) ;

ابعاد

24 cm

عنوان فروست

Methods in enzymology,

مشخصه جلد

volume 546

شاپا ي ISSN فروست

0076-6879 ;

متن يادداشت

Includes bibliographical references and indexes

متن يادداشت

In vitro enzymology of Cas9 -- Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs -- Determining the specificities of TALENs, Cas9, and other genome-editing enzymes -- Genome engineering with custom recombinases -- Genome engineering in human cells -- Genome editing in human stem cells -- Tagging endogenous loci for live-cell fluorescence imaging and molecule counting using ZFNs, TALENs, and Cas9 -- Genome editing using Cas9 nickases - Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases -- Adapting CRISPR/Cas9 for functional genomics screens -- The iCRISPR platform for rapid genome editing in human pluripotent stem cells -- Creating cancer translocations in human cells using Cas9 DSBs and nCas9 paired Nicks -- Genome editing for human gene therapy -- Generation of site-specific mutations in the rate genome via CRISPR/Cas9 -- CRISPR/Cas9-based genome editing in mice by single plasmid injection -- Imaging genomic elements in living cells using CRISPR/Cas9 -- Cas9-based genome editing in xenopus tropicalis -- Cas9-based genome editing in zebrafish -- Cas9-based genome editing in drosophila -- Transgene-free genome editing by germline injection of CRISPR/Cas RNA -- Cas9-based genome editing in arabidopsis and tobacco -- Mutliplex engineering of industrial yeast genomes using CRISPRm -- Protein engineering of Cas9 for enhanced function

بدون عنوان

0

موضوع مستند نشده

Endonucleases, Laboratory manuals

موضوع مستند نشده

Genetic engineering, Laboratory manuals

موضوع مستند نشده

Genomics, Laboratory manuals

موضوع مستند نشده

Restriction enzymes, DNA, Laboratory manuals

شماره

ويراست

شماره رده

نشانه اثر

مستند نام اشخاص تاييد نشده

Doudna, Jennifer A.

مستند نام اشخاص تاييد نشده

Sontheimer, Erik J.

تاريخ عمليات

20160712084354.0

قواعد فهرست نويسي ( بخش توصيفي )

rda

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y