عنوان

The genetic manipulation of staphylococci

شابک

9781493931576 )alk. paper(

عنوان اصلي

The genetic manipulation of staphylococci

نام نخستين پديدآور

edited by Jeffrey L. Bose, University of Kansas Medical Center, Kansas City, KS, USA

محل نشرو پخش و غیره

New York

نام ناشر، پخش کننده و غيره

Springer

تاریخ نشرو بخش و غیره

2016

نام خاص و کميت اثر

x, 186 pages : illustrations

عنوان فروست

Springer protocols

عنوان فروست

Methods in molecular biology,1064-3745 ;1373

عنوان فروست

Series: Springer protocols )Series(,1949-2448

عنوان فروست

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1373,1064-3745

متن يادداشت

Understanding staphylococcal nomenclature /Christina N. Krute and Jeffrey L. Bose --Restriction-modification systems as a barrier for genetic manipulation of Staphylococcus aureus /Marat R. Sadykov --Complementation plasmids, inducible gene-expression systems, and reporters for Staphylococci /Ralph Bertram --De novo assembly of plasmids using yeast recombinational cloning /Ameya A. Mashruwala and Jeffrey M. Boyd --Splicing by overlap extension PCR to obtain hybrid DNA products /Justin A. Thornton --Method for preparation and electroporation of S. aureus and S. epidermidis /Melinda R. Grosser and Anthony R. Richardson --Rapid isolation of DNA from staphylococcus /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus : broth-based method /Kelsey L. Krausz and Jeffrey L. Bose --Bacteriophage transduction in Staphylococcus aureus /Michael E. Olson --Batch transduction of transposon mutant libraries for rapid phenotype screening in staphylococcus /Katherine L. Maliszewski --Conjugative transfer in Staphylococcus aureus /Cortney R. Halsey and Paul D. Fey --Allelic exchange /McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles --Creation of staphylococcal mutant libraries using transposon Tn917 /Kelly C. Rice --Generation of a transposon mutant library in Staphylococcus aureus and staphylococcus epidermidis using bursa aurealis /Vijaya Kumar Yajjala ]and others[ --Chemical and UV mutagenesis /Jeffrey L. Bose --Pulse field gel electrophoresis /Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler Jr. --RNA-sequencing of Staphylococcus aureus messenger RNA /Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw --Quantitative real-time PCR )qPCR( workflow for analyzing Staphylococcus aureus gene expression /April M. Lewis and Kelly C. Rice --Electrophoretic mobility shiftaAssays /Sarah E. Rowe and James P. O'Gara --Rapid amplification of cDNA ends for RNA transcript sequencing in staphylococcus /Eric Miller.

متن يادداشت

Includes bibliographical references and index.

عنوان گونه گون

methods and protocols

عنصر شناسه ای

Staphylococcus^agenetics

عنصر شناسه ای

Genetic Phenomena

عنصر شناسه ای

Genetic Techniques

عنصر شناسه ای

Laboratory Manuals

شماره رده

شماره رکورد رده بندي

نشانه اثر

شماره رکورد غير از شماره رده بندي

عنصر شناسه اي

Bose, Jeffrey L.

نوع ماده

English Book