Methods in molecular biology (Clifton, N.J.), v. 1678.
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Includes bibliographical references and index
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Dedication...Preface...Table of Contents...Contributing Authors....1. Flow Cytometry:- The Glass is Half Full Howard M. Shapiro2. High-Dimensional Modeling for Cytometry: Building Rock Solid Models using GemStoneTM and Verity Cen-se'TM High-Definition t-SNE Mapping C. Bruce Bagwell3. Mass Cytometry Assays for Antigen-Specific T Cells using CyTOFDongxia Lin and Holden T. Maecker4. RNA Flow Cytometry using the Branched DNA TechniqueKah Teong Soh and Paul K. Wallace5. Analysis of Individual Extracellular Vesicles by Flow CytometryJohn P. Nolan and Erika Duggan6. Quantitative Fluorescence Measurements with Multicolor Flow CytometryLili Wang, Adolfas K. Gaigalas, and James Wood7. High Throughput Flow Cytometry for Cell Surface ProfilingJoshua Paterson and Laurie E. Ailles8. Multiparameter Conventional Flow CytometryKatherine M. McKinnon9. Multiparameter Intracellular Cytokine StainingPatricia Lovelace and Holden T. Maecker10. Multiparameter Analysis of Apoptosis by Flow CytometryWilliam G. Telford11. Multiparameter Cell Cycle AnalysisJames W. Jacobberger, R. Michael Sramkoski, Tammy Stefan, and Philip G. Woost12. Monitoring Cell Proliferation by Dye Dilution: Considerations for Probe SelectionJoseph D. Tario, Jr., Alexis N. Conway, Katharine A. Muirhead, and Paul K. Wallace13. Immunophenotypic Identification of Early Myeloerythroid DevelopmentCornelis J. H. Pronk, and David Bryder14. Flow Cytometry Assays in Primary Immunodeficiency DiseasesMaurice R. G. O'Gorman15. Real-Time Deformability Cytometry: Label Free Functional Characterization of CellsMaik Herbig, Martin Krater, Katarzyna Plak, Paul Muller, Jochen Guck, and Oliver Otto16. Nuclear Cytometry: Analysis of the Patterns of DNA Synthesis and Transcription using Flow Cytometry, Confocal Microscopy, and RNA SequencingDavid W. Galbraith, Elwira Sliwinska, and Partha Samadder17. Flow Cytometric FRET Analysis of Protein InteractionsLaszlo Ujlaky-Nagy, Peter Nagy, Janos Szoellosi, and Gyoergy Vereb18. Overview of Fluorescence Lifetime Measurements in Flow CytometryJessica P. Houston, Zhihua Yang, Jesse Sambrano, Wenyan Li, Kapil Nichani, and Giacomo Vacca19. Overview of Lasers for Flow CytometryWilliam G. Telford20. Flow Cytometry: The Glass is Half EmptyHoward M. Shapiro.
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This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition is a valuable resource for researchers and scientists who are interested in continuing and/or expanding their knowledge of flow cytometry.