عنوان

Neurocytochemical Methods

3642842984

364284300X

9783642842986

9783642843006

b574527

Neurocytochemical Methods

[Book]

edited by André Calas, Daniel Eugène.

Berlin, Heidelberg

Springer Berlin Heidelberg

1991

NATO ASI series., Series H,, Cell biology ;, 58.

Development and application of antibodies to primary (DA, L-DOPA) and secondary (cGMP) messengers: A technical report --; Hodology --; Transmitter-related retrograde labeling and immunohistochemistry to localize amino acid transmitters in neural pathways and cells --; Ultrastructural identification and quantification of neuroactive amino acids --; Video-microscopic techniques to study axonal transport, neuronal fine structure and cytochemical parameters in live neurons --; Investigating the three-dimensional morphology of fluorescence-labelled neurons using confocal laser scanning microscopy --; Chairman's report --; Detection of Specific Molecules --; Combined cytolabeling methods for investigating chemo-specific neuronal interactions and transmitter co-localization --; Molecular neuroanatomy of synapses, cells and systems in the brain --; Regulated expression of transcription factor and neurotransmitter genes in neural populations: studies using in situ hybridization with radiolabeled oligonucleotide probes --; The use of markers to study the regulation of glial cells in the PNS and CNS --; Chairlady's report --; Quantitative Aspects --; Applications to the study of acetylcholine release of the choline oxidase acetylcholine assay --; Morphometric evaluation of populations of neuronal profiles in the central nervous system --; Positron emission tomography: functional neuroanatomy in vivo --; Microfluorometric imaging of time and space dependent calcium concentration changes in mammalian neurons --; Chairman's report --; Receptors --; Immunocytochemistry of neuropeptide receptors using anti-idiotypic antibodies and antibodies to peptides encoded by complementary RNA --; New approaches for receptor analysis: histochemistry of the GABA/BZ receptors in the rat CNS --; Chairman's report --; Short Papers --; Extracellular dyes induce photodegeneration and photopermeabilization of vertebrate and invertebrate neurons --; A method to study projections of GABAergic neurons using deletion of the GABA immunostaining by metallic ions --; Ultrastructural immunocytochemical localization of choline acetyltransferase in sympathetic ganglia --; A dual immunoperoxidase labelling method to study the ultrastructural relationships between choline acetyltransferase- and neuropeptide Y-containing neurons in the rat striatum --; Characterization of microglia and brain macrophages --; Visualization and quantification of 5-HT1A receptors in the rat brain by radioimmunohistochemistry --; Anti-idiotypic antibodies against opioid receptors: first ultrastructural evidence for a presynaptic inhibition by opioids in the dorsal horn of the rat spinal cord --; Antibodies to complementary peptide for labelling substance P receptors in the dorsal horn of the rat spinal cord for both light and electron microscopy --; Comparative ultrastructural localization of neuropeptide mRNAs using tritiated and biotinylated oligodeoxynucleotide probes --; Use of in situ hybridization and immunohistochemistry to study the effects of intranigral injections of colchicine on enkephalin- and substance P-containing neurons of the limbic forebrain --; Chairman's report --; Author Index.

The book is aimed at providing researchers, postgraduate students, neurologists, and biological laboratory staff with accurate and up-to-date descriptions of most recent and powerful neurocytochemical methods: localization and co-localization of neurotransmitters; study of their cellular and sub-cellular interactions; histological and ultrastructural detection of receptors; quantification of mediators;hodology and 3-D reconstruction of neurons; cytofunctional approach of axonal transport, of genic expression, and of physiological effects of neurotransmitters (PET, voltage- or ion-sensitive dyes). The possibilities limits, technical difficulties, and applications of these techniques and of their possible combinations on the same tissue are emphasized.

Proceedings of the NATO Advanced Research Workshop on Recent Advances in Neurocytochemical Methods held in Seillac, France, September 26-28, 1990

Human physiology.

Medicine.

Neurosciences.

edited by André Calas, Daniel Eugène.

André Calas

Daniel Eugène

[Book]

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