عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
INTERNATIONAL STANDARD BOOK NUMBER
(Number (ISBN
978-1-4939-8860-0
(Number (ISBN
978-1-4939-8861-7 (eBook)
NATIONAL BIBLIOGRAPHY NUMBER
Number
6042
LANGUAGE OF THE ITEM
.Language of Text, Soundtrack etc
انگلیسی
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
BCL-2 family proteins: methods and protocols
General Material Designation
[electronic resources]
First Statement of Responsibility
/ edited by Evripidis Gavathiotis.
.PUBLICATION, DISTRIBUTION, ETC
Place of Publication, Distribution, etc.
New York, NY
Name of Publisher, Distributor, etc.
: Springer Science+Business Media
Date of Publication, Distribution, etc.
, 2019.
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
xi, 360 pages.
Other Physical Details
: illustrations (color), tables, charts.
SERIES
Series Title
Springer protocols.; Methods in molecular biology (Clifton, N.J.), 1877.
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
Text of Note
Includes bibliographical references.
CONTENTS NOTE
Text of Note
Overview of BCL-2 family proteins and therapeutic potentials / Jason D. Huska, Heather M. Lamb, and J. Marie Hardwick --Database and bioinformatic analysis of BCL-2 family proteins and BH3-only proteins / Abdel Aouacheria, Vincent Navratil, and Christophe Combet --Application of mito-priming to generate BCL-2 addicted cells / Jonathan Lopez and Stephen W.G. Tait --BH3 profiling : a functional assay to measure apoptotic priming and dependencies / Cameron Fraser, Jeremy Ryan, and Kristopher Sarosiek --Flow cytometry-based detection and analysis of BCL-2 family proteins and mitochondrial outer membrane permeabilization (MOMP) / Lindsey M. Ludwig, Katrina L. Maxcy, and James L. LaBelle --Investigating BCL-2 family protein interactions in yeast / Steþphen Manon --Liposomal permeabilization assay to study the functional interactions of the BCL-2 / Denis E. Reyna and Evripidis Gavathiotis --Mitochondrial isolation and real-time monitoring of MOMP / Mark P.A. Luna-Vargas, Jarvier N. Mohammed, Jesse D. Gelles, and Jerry Edward Chipuk --Photocrosslinking approach to investigate protein interactions in the BCL-2 family / Jialing Lin, Arthur E. Johnson, and Zhi Zhang --Assessment of dynamic BCL-2 protein shuttling between outer mitochondrial membrane and cytosol / Joachim Lauterwasser, Franziska Fimm-Todt, and Frank Edlich --Quantification of BCL-2 family members by flow cytometry / Morey L. Smith and Stephen K. Tahir --Methods to probe calcium regulation by BCL-2 family members / Marcos A. Carpio and Samuel G. Katz --Methods to probe conformational activation and mitochondrial activity of proapoptotic BAK / Geetika Singh and Tudor Moldoveanu --Probing BAK and BAX activation and pore assembly with cytochrome c release, limited proteolysis, and oxidant-induced linkage / Sweta Iyer, Rachel T. Uren, and Ruth M. Kluck --BCL-2 protein family interaction analysis by nuclear magnetic resonance spectroscopy / Thomas P. Garner and Evripidis Gavathiotis --Reconstitution and characterization of BCL-2 family proteins in lipid bilayer nanodiscs / Yong Yao and Francesca M. Marassi --Cryo-electron microscopy to study Bax pores and MOMP / Tomomi Kuwana --CW EPR and DEER methods to determine BCL-2 family protein structure and interactions : application of site-directed spin labeling to BAK apoptotic pores / Tirtha Mandal, Eric J. Hustedt, Likai Song, and Kyoung Joon Oh --Rapid imaging of BCL-2 family interactions in live cells using FLIM-FRET / Elizabeth J. Osterlund, Nehad Hirmiz, Christian Tardif, and David W. Andrews --Quantification of the interactions between BCL-2 proteins by fluorescence correlation spectroscopy / Fabronia Murad and Ana J. Garcia-Saez --Isolation of synthetic antibodies against BCL-2-associated X protein (BAX) / Zhou Dai and Jonathan R. Lai.
0
SUMMARY OR ABSTRACT
Text of Note
This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, BCL-2 Family Proteins: Methods and Protocols is a valuable resource to inspire and encourage novice and established scientists to further their research and make new discoveries in this exciting field.
TOPICAL NAME USED AS SUBJECT
Entry Element
Proto-Oncogene Proteins c-bcl-2--metabolism.
Entry Element
Bcl-2 protein family.
UNCONTROLLED SUBJECT TERMS
Subject Term
Proto-Oncogene Proteins.
PERSONAL NAME - SECONDARY RESPONSIBILITY
Relator Code
Editor
Gavathiotis, Evripidis,
ORIGINATING SOURCE
Country
ایران
Agency
Central Library of the Kurdistan University
Date of Transaction
20181015102016.0
Cataloguing Rules (Descriptive Conventions))
rda
e
BL
280328
a
Y
