تنوع ژنتیکی و ساختار جمعیت خویشاوندان وحشی گندم بر اساس نشانگرهای SSR گندم و جو Genetic diversity and population structure of wheat wild relatives based on wheat and barley SSR markers
عنوان اصلي به زبان ديگر
SB
نام نخستين پديدآور
/احمد احمدی لکی
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
: دانشکده کشاورزی
تاریخ نشرو بخش و غیره
، ۱۳۹۴
یادداشتهای مربوط به نشر، بخش و غیره
متن يادداشت
چاپی
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
کارشناسی ارشد
نظم درجات
رشته اصلاح نباتات
زمان اعطا مدرک
۱۳۹۲/۰۶/۱۸
کسي که مدرک را اعطا کرده
تبریز
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Wheat wild relatives with genes conferring resistance to biotic and abiotic stresses are valuable genetic resources to utilize in breeding programs. Determination of genetic structure and relationships of these resources is essential for their utilization. In this study, genetic diversity and population structure of 40 genotypes belonging to nine wheat wild relatives was investigated using 226 wheat and 212 barley SSR markers. Out of 438 used primer pairs, 120 were amplified in the studied genotypes and finally, 100 polymorphic primer pairs (50 wheat and 50 barley SSR markers) with proper banding pattern were used for diversity and population structure analyses. By using 50 wheat SSR primer pairs, 205 alleles were amplified in the studied genotypes and number of alleles varied from 2 to 12 with an average of 4.1 allele per locus. Mean gene diversity and polymorphism information content (PIC) were 0.53 and 0.48, respectively. In total, 50 barley primer pairs amplified 223 alleles in wheat wild relatives. Number of alleles ranged from 2 and 14 with mean of 4.46 alleles per locus and mean gene diversity and PIC for these markers were 0.6 and 0.55, respectively. In the analysis of molecular variance using wheat SSR data, 52 and 48 of total molecular variation was explained by between and within species variances, respectively. These amount for barley SSR markers were 40 and 60 and for the combination of wheat and barley SSRs were 39 and 61 , respectively. Cluster analysis using Neighbor-Joining algorithm and Juckes Cantor distance coefficient based on molecular data assigned the studied genotypes into fine groups according to their genomic composition. In the principal coordinate analysis based on SSR data, the first coordinates explained more than 60 of the total molecular variation of the genotypes. Using two first coordinates, the genotypes were grouped according to their genome, similar to the cluster analysis. Based on the estimated genetic parameters, maximum and minimum within species variation were observed in Ae. ambellulata and T. urartu, respectively
عنوان اصلی به زبان دیگر
عنوان اصلي به زبان ديگر
SB
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )