عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304354349
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
The human MUC5 gene and its hamster homologue:
نام عام مواد
[Thesis]
نام نخستين پديدآور
D. M. Meerzaman
عنوان اصلي به قلم نويسنده ديگر
Studies of cloning, characterization, tissue expression and regulation by retinoic acid
نام ساير پديدآوران
M. C. Rose
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
The George Washington University
تاریخ نشرو بخش و غیره
1997
مشخصات ظاهری
نام خاص و کميت اثر
194
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
The George Washington University
امتياز متن
1997
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Mucus is a viscoelastic gel-like component that protects and lubricates mammalian epithelia. The major component of mucus is a family of glycoproteins known as mucins which are heavily glycosylated, large molecular weight molecules that contribute to the viscosity of mucus. Their protein backbones are encoded by mucin genes. In order to identify the mucin gene that encoded tracheobronchial mucin (TBM), a nasal polyp cDNA library was generated and screened with a unique nucleotide probe that encoded the TR-3A peptide sequence of TBM. A 3567 bp clone, NP3a, was identified and shown to encode the usd3\primeusd end of the MUC5 gene. It encodes a five amino acid repeat unit tandemly repeated five times and two octapeptides that are conserved among several human and animal secretory mucin genes. The NP3a gene was localized to chromosome 11. To obtain additional information about the structure of MUC5, a 34 kb genomic MUC5 clone, CRIE1, was subjected to restriction digestion. An EcoRI/XhoI 5.5kb fragment was subcloned and sequenced revealing almost perfect identity with 650bp located at the extreme usd3\primeusd end of clone NP3a. The regulation of MUC5 expression by retinoic acid was examined in vitro using A549 cells, a lung carcinoma cell line. MUC5 expression was shown to be increased in both a time and dose-dependent manner. MUC5 regulation was also investigated in vivo using a hamster model. A 534bp hamster Muc-5 homologue was isolated from hamster stomach cDNA by RT-PCR and subcloned. Sequence analysis revealed a high degree of homology between the human MUC5 and rat Muc-5. Using the hamster cDNA clone as probe, Northern blot analysis revealed an increase in Muc-5 expression in RNA isolated from the gastro-intestinal tract and trachea of Golden Syrian hamsters fed a diet enriched in retinoic acid. Northern blot analysis demonstrated that both human MUC5 and hamster Muc-5 showed high levels of gene expression in stomach. In addition, NP3a showed a significant degree of MUC5 expression in upper respiratory tract tissue.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Cellular biology
موضوع مستند نشده
Genetics
موضوع مستند نشده
mucin
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
D. M. Meerzaman
مستند نام اشخاص تاييد نشده
M. C. Rose
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
