عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304321590
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Study of a tomato protein which inhibits endo-polygalacturonase from Verticillium albo-atrum
نام عام مواد
[Thesis]
نام نخستين پديدآور
L.-K. Huang
نام ساير پديدآوران
R. R. Mahoney
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Massachusetts Amherst
تاریخ نشرو بخش و غیره
1996
مشخصات ظاهری
نام خاص و کميت اثر
307
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Massachusetts Amherst
امتياز متن
1996
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
A polygalacturonase-inhibiting protein (PGIP) was purified from tomato fruit and stem tissue and was subsequently characterized. PGIP was found in stem, leaf and fruit tissue of tomato but not in root tissue. The PGIP was purified by a combination of ion-exchange, gel filtration and hydrophobic interaction chromatography. The purified tomato fruit PGIP appeared on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a broad band with a molecular weight range between 37 kDa and 43 kDa. Its molecular weight as estimated by gel filtration was 43 kDa. It stained positive for the presence of carbohydrate and had a pI of greater than 8.6. The PGIP was active against Verticillium albo-atrum endo-polygalacturonase (endo-PG) and, to a lesser extent, Aspergillus niger endo-PG. It did not inhibit crude endo-PG preparations from several other fungi or tomato endo-PG. The PGIP was stable in the pH range 3-13 and its activity was essentially constant in the pH range 3.8-6.9. It was stable at temperatures up to 50C. Preincubation of PGIP with endo-PG before adding substrate solution enhanced PGIP activity. The endo-PG from V. albo-atrum was purified to electrophoretic homogeneity on SDS-PAGE. The enzyme was capable of hydrolyzing several pectic substrates, including trigalacturonate, polygalacturonate, pectin with 31%, 56%, 70% and 93% esterification, and pectin in cell walls from tomato fruit. Non-denaturing PAGE and affinity chromatography were used to study the interaction of the enzyme and tomato PGIP. In both systems, it was demonstrated that the substrate, i.e., polygalacturonate, was required for the formation of an enzyme-inhibitor complex. In the presence of tomato PGIP, endo-PG acted on polygalacturonate and tomato cell walls in a similar way but at a much slower pace. Consequently, the presence of oligogalacturonides which can act as phytoalexin elicitors was extended.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Aspergillus niger
موضوع مستند نشده
Biochemistry
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
phytoalexin
موضوع مستند نشده
Plant pathology
موضوع مستند نشده
Pure sciences
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
L.-K. Huang
مستند نام اشخاص تاييد نشده
R. R. Mahoney
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
