عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq193297408
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Characterization of cardiotonic extracts of sea anemones from the west coast of Canada
نام عام مواد
[Thesis]
نام نخستين پديدآور
E. I. Cline
نام ساير پديدآوران
L. I. Wiebe
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Alberta (Canada)
تاریخ نشرو بخش و غیره
1996
مشخصات ظاهری
نام خاص و کميت اثر
261
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Alberta (Canada)
امتياز متن
1996
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Eleven sea anemones species (Anthopleura elegantissima, A. xanthogrammica, Corynactis californica, Epiactis prolifera, Metridium senile, Pachycerianthus fimbratus, Stomphia didemon, Urticina coriacea, U. crassicornis, U. lofotensis and U. piscivora), collected from the west coast of Canada, were screened for cardiotonic, cytolytic, cytotoxic and antifungal activities. Extracts from nine species elicited cardiotonic activity on rat atria. While P. fimbratus was inactive, E. prolifera was cardiotoxic. Eight extracts were hemolytic on erythrocytes of rat, guinea pig and dog; six exhibited cytotoxic activity to KB, L1210 and HEL 299 cell lines. Extracts from all anemone species were devoid of antifungal activity against Aspergillus niger PLM 1140 and Candida albicans ATCC 14053. From extracts of U. piscivora, three cardiotonic proteins (UpI, UpII and UpIII) were purified using a combination of chromatographic methods. UpI is a basic protein, (pI > 9.3), of molecular weight 28 kDa, and N-terminal amino acid sequence of usd\rm D\sp1ENEN\sp5LYGPN\spENKAK\spAKDLT\sp{20}AGASY\sp{25}LT KEA\sp{30}GCT KL\sp{35}QAGCT\sp{40}MYQAY\sp{45}Nusd. It elicited potent cardiac stimulatory activity on rat left atria (EC{50}: usd8.1\times 10\sp{-9}usd M) comparable to isoproterenol (EC{50}: usd3\times 10\sp{-9}usd M). Although different from known anemone proteins, UpI showed some sequence similarity to the bungarotoxins from Bungarus multicintus. UpII and UpIII (pI = 7.2 and 7.6, respectively) appear to be variants of each other with similar molecular weights of 19 and 40 kDa in reducing and non-reducing conditions, respectively. Their N-terminal amino acid sequences were usd\rm A\sp1TDKW\sp5NDCG S\spVTALC\spEQKGF\sp{20}NKATC\sp{25}usd (UpII), and usd\rm D\sp1DDWD\sp5EGCHV\spTALLEGQQGR\sp{20}NK AAC\sp{25}usd (UpIII). UpII (ED{50}: usd1\times 10\sp{-7}usd M) was more potent on rat left atria than UpIII (ED{50}: usd7\times 10\sp{-6}usd M). UpI was hemolytic to erythrocytes of rat, guinea pig, dog, pig and human. Using scanning electron microscopy, it was shown to cause structural damage to the membranes of rat and guinea pig erythrocytes. Hemolysis was inhibited by sphingomyelin. UpII and UpIII were not hemolytic. Pathophysiological investigations of all proteins revealed only UpI to be toxic, causing hypotension, respiratory and circulatory failure in anesthetized rats, similar to the crude extract from U. piscivora. Autopsy revealed morphological damage to the skin, lungs, liver and kidney, but not the heart and brain. All three proteins were devoid of antifungal activity. While UpI appears to belong to the class of toxins with phospholipase A activity, UpII and UpIII appear to be a new class of cardiotonic proteins.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Anthopleura
موضوع مستند نشده
Corynactis californica
موضوع مستند نشده
Epiactis prolifera
موضوع مستند نشده
Health and environmental sciences
موضوع مستند نشده
Metridium senile
موضوع مستند نشده
Pachycerianthus fimbratus
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
E. I. Cline
مستند نام اشخاص تاييد نشده
L. I. Wiebe
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
