عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304446474
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
Modification of vegetable soybean sugar content via gene transformation
نام عام مواد
[Thesis]
نام نخستين پديدآور
S. Chanprame
نام ساير پديدآوران
J. M. Widholm
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Illinois at Urbana-Champaign
تاریخ نشرو بخش و غیره
1998
مشخصات ظاهری
نام خاص و کميت اثر
97
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
University of Illinois at Urbana-Champaign
امتياز متن
1998
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
In a preliminary study, somatic and zygotic embryos of soybean cv. Jack were analyzed for soluble carbohydrate, total lipids and protein during development. These studies demonstrated that the composition of Jack zygotic embryos is similar to that described for other cvs. during development while the somatic embryo composition and size is markedly different. Glucose, sucrose and the usd\alphausd-galactoside stachyose are found in somatic embryos. These studies have provided provide information concerning the abnormal biochemical changes occurring during development of somatic embryos that might help improve plant regeneration. We can also monitor the effect of genetic engineering approaches that might modify the seed composition using somatic embryos rather than seeds produced after plant regeneration. To modify soybean sugar content, somatic embryos of soybean cvs. Peterson Jade, Daelip Hakja (PI 157.419), Yakumo Meaka (PI 417.468), Kinshu (PI 549.065) Kaohsiung No.1 (PI 536.546) and Jack were transformed with yeast invertase (SUC 2) and usd\alphausd-galactosidase (agl A) from Aspergillus niger under the control of the lectin promoter using particle bombardment. The transformation was not very successful. In some cultivars, no green clumps were obtained after 8 weeks of selection in FG medium containing 25 mug/ml hygromycin B. The green clumps obtained from transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or usd\alphausd-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost during the somatic embryo multiplication due to the friability of some green clumps that were discarded during the culture process.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Alpha-galactosidases
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Gene transformation
موضوع مستند نشده
Glycine max
موضوع مستند نشده
Soybean
موضوع مستند نشده
Sugar
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
J. M. Widholm
مستند نام اشخاص تاييد نشده
S. Chanprame
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
