عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TLpq304083339
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
A study of the structure of the Bacillus subtilis 168 chromosome
نام عام مواد
[Thesis]
نام نخستين پديدآور
M. Amjad
نام ساير پديدآوران
P. J. Piggot
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Temple University
تاریخ نشرو بخش و غیره
1993
مشخصات ظاهری
نام خاص و کميت اثر
152
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
کسي که مدرک را اعطا کرده
Temple University
امتياز متن
1993
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
An SfiI restriction map of the spore-forming bacterium Bacillus subtilis 168 was constructed. Twenty-three SfiI fragments were resolved by pulsed-field gel electrophoresis. These fragments ranged in size from 730 kb to 7 kb. The order of the fragments on the B. subtilis genome was deduced by a variety of methods. Extensive use was made of Southern blots using cloned, genetically mapped DNA fragments as probes. Integrative plasmids were used to distinguish fragments of similar size. Linking clones were used to determine the contiguity of 9 pairs of SfiI fragments. Partial SfiI digestion analysis was used to locate smaller fragments. Seventy cloned genetic markers were located on 19 SfiI fragments. Using these and other methods a complete SfiI restriction map was constructed. The size of the B. subtilis genome was estimated to be 4188 kb, 200 kb. The resulting physical map was used for mapping newly cloned DNA on the B. subtilis chromosome. Taking advantage of the physical map generated in this study, a general search was conducted in order to study chromosomal DNA rearrangements during sporulation. A study of SfiI restriction patterns of chromosomal DNA isolated from vegetative and sporulating bacteria confirmed the presence of about a 42 kb deletion in the region of spoIIIC; in fragment SF4. Using probes for cloned genes, representing 18 SfiI fragments, no other rearrangements were detected similar to the change in fragment SF4. A possible role of DNA gyrase (encoded by the gyrA, and gyrB genes) in nucleoid condensation during sporulation was investigated. In a series of experiments, no strong link was established between nucleoid condensation and transcription of either gyrA or gyrB.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biological sciences
موضوع مستند نشده
Genetics
موضوع مستند نشده
Molecular biology
موضوع مستند نشده
Molecular biology
موضوع مستند نشده
nucleoid condensation
موضوع مستند نشده
sporulation
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
M. Amjad
مستند نام اشخاص تاييد نشده
P. J. Piggot
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
