عنوان

Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae

شماره

TLpq304057231

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

Further characterization of tRNA(Trp) genes of Saccharomyces cerevisiae

نام عام مواد

[Thesis]

نام نخستين پديدآور

M. A. Riazi

نام ناشر، پخش کننده و غيره

University of Alberta (Canada)

تاریخ نشرو بخش و غیره

1992

نام خاص و کميت اثر

118

جزئيات پايان نامه و نوع درجه آن

M.Sc.

کسي که مدرک را اعطا کرده

University of Alberta (Canada)

امتياز متن

1992

متن يادداشت

Two goals were pursued in this work. First, attempts were made to construct an intronless ochre suppressor tRNATrp gene. The ability of the construct to function as an ochre suppressor was studied in Saccharomyces cerevisiae in vivo. The in vitro-constructed intronless tRNATrp gene suppressed the tested ochre mutations. This result supported models emphasizing the importance of the intron-anticodon stem loop binding in the splicing of pre-tRNA transcripts. These interactions are supposedly disrupted after a CCA to TTA change in the anticodon of the putative suppressor tRNATrp gene. An intronless gene would not have this problem since the need for splicing is abolished. The other interesting aspect of this work was the effect of an extrachromosomal factor (usd\Psi\sp{+}usd). The ability of the constructed tRNATrp gene to suppress one of the tested ochre mutations (lys1-1) was significantly increased in the presence of this factor, and reduced when this factor was eliminated. In the second part of this work the 5 and 3 flanking regions of the six tRNATrp genes in Saccharomyces cerevisiae were studied. (Abstract shortened by UMI.)

موضوع مستند نشده

Biological sciences

موضوع مستند نشده

Genetics

مستند نام اشخاص تاييد نشده

M. A. Riazi

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y