عنوان

DR5-AS uzun kodlamayan RNA'sının hücre proliferasyonu üzerine olan etkisinin araştırılması

شماره

TLpq2522841702

زبان متن نوشتاري يا گفتاري و مانند آن

انگلیسی

عنوان اصلي

DR5-AS uzun kodlamayan RNA'sının hücre proliferasyonu üzerine olan etkisinin araştırılması

نام عام مواد

[Thesis]

نام نخستين پديدآور

Gürer, Dilek Cansu

نام ساير پديدآوران

Akgül, Bünyamin

نام ناشر، پخش کننده و غيره

Izmir Institute of Technology (Turkey)

تاریخ نشرو بخش و غیره

2020

نام خاص و کميت اثر

63

جزئيات پايان نامه و نوع درجه آن

Master's

کسي که مدرک را اعطا کرده

Izmir Institute of Technology (Turkey)

امتياز متن

2020

متن يادداشت

Cell proliferation is the process of increasing cell number in a multicellular organism. In literature, there are numerous proteins and non-coding RNAs reported as regulators of cell proliferation, yet, many of others are waiting to be explored. Unravelling the mechanism behind the regulation of cell proliferation is crucial to develop new strategies for fighting numerous diseases such as cancer, immune diseases, or neurodegenerative diseases. Long non-coding RNAs (lncRNAs) are known to regulate various cellular processes. To determine which ones are related to cell proliferation and apoptosis in HeLa cells, a transcriptomics study was performed under cisplatin, doxorubicin, TNF-α and Anti-Fas treatments. DR5-AS is a novel lncRNA transcript selected from this transcriptomics study as a promising regulatory lncRNA candidate due to its overlap with DR5 protein-coding gene which is known to regulate apoptosis and proliferation. Several phenotypic characterization methods were performed to understand the function of DR5-AS lncRNA. These studies showed that DR5-AS knockdown causes a significant decrease in cell proliferation, an alteration in the normal HeLa cell morphology, a shift through S and G2/M phases in cell cycle profile, and significant accumulation of cells in the metaphase phase. A second transcriptomics study was performed with DR5-AS knockdown HeLa cells to uncover which pathways are responsible for these changes. The results suggest that DR5-AS lncRNA regulates expression of numerous key proteins in cell cycle regulation. This observation was confirmed by several qPCR experiments. In conclusion, this study provides the first evidence that DR5-AS lncRNA modulates cell cycle and proliferation in HeLa cells.

موضوع مستند نشده

Cancer

موضوع مستند نشده

Genetic engineering

موضوع مستند نشده

Genetics

موضوع مستند نشده

Molecular biology

مستند نام اشخاص تاييد نشده

Akgül, Bünyamin

مستند نام اشخاص تاييد نشده

Gürer, Dilek Cansu

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y