عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL50741
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
The Influence of Alcohol on Telomere Length and Shelterin Complex Gene Expression in Human Embryonic Stem Cells
نام عام مواد
[Thesis]
نام نخستين پديدآور
Moazzam, Muhammad Talha
نام ساير پديدآوران
Zhang, Huiping
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
Boston University
تاریخ نشرو بخش و غیره
2019
يادداشت کلی
متن يادداشت
80 p.
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
M.S.
کسي که مدرک را اعطا کرده
Boston University
امتياز متن
2019
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Background: Telomeres are specialized heterochromatic structures found at the ends of chromosomes that serve to protect the integrity of the genome. Theoretically, telomeres, and thereby chromosomes, should be constantly shortening however that is not the case. Telomere length homeostasis is maintained via the activity of the enzyme Telomerase as well as a complex of 6 proteins, called the shelterin complex. Decreased telomere length is associated with a variety of neuropsychological diseases such as Alzheimer's disease, and chronic depression. Objective: The primary objective was to understand the effect of alcohol exposure on telomere dynamics, specifically on telomere length as well as the expression of the shelterin complex genes. This was accomplished using human embryonic stem cells (hESCs) as models. This study was exploratory with the goal of increasing our knowledge of genes involved in telomere maintenance. Methods: hESCs were cultured in cell culture media containing 25 mM, 50 mM, or 100 mM of ethanol. After a 3-, 7-, or 14-day ethanol exposure plus a 24-hour withdrawal, hESCs were collected and genomic DNAs and total RNAs was extracted. Ethanol-induced telomere length changes were examined by quantitative polymerase chain reactions (qPCR).Ethanol-induced shelterin complex gene expression changes were examined by reverse-transcription and quantitative polymerase chain reactions (RT-qPCRs). Results: Extended alcohol exposure exerted a deleterious effect on telomeres, causing them to diminish in length. The effects of alcohol on the expression of shelterin complex genes were varied, ranging from consistent upregulation or downregulation to a combination of both. The concentration of alcohol was inversely correlated with the expression of shelterin complex genes, and the correlation was influenced by the duration of ethanol exposure. Conclusion: Chronic exposure to alcohol resulted in the shortening of telomeres. The effect ethanol was both time- and concentration-dependent, with telomere length having a negative relationship with the concentration of ethanol as well as the duration of exposure. Additionally, the shelterin subunit genes had their expression levels altered by ethanol in a manner which was consistent with what has been observed in prior studies as well as what aligns with their respective functions.
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Genetics
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Moazzam, Muhammad Talha
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Zhang, Huiping
شناسه افزوده (تنالگان)
مستند نام تنالگان تاييد نشده
Boston University
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
