عنوان

Cytoskeleton methods and protocols /

شابک

0896037711

شابک

1280820438

شابک

1592590519

شابک

6610820430

شابک

9780896037717

شابک

9781280820434

شابک

9781592590513

شابک

9786610820436

شابک اشتباه

9781617371813

شماره

b763024

عنوان اصلي

Cytoskeleton methods and protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Ray H. Gavin.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2001.

نام خاص و کميت اثر

1 online resource (xiv, 288 pages) :

ساير جزييات

illustrations (some color)

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 161

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

Identification of Cytoskeleton Proteins -- Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments -- Microsequencing of Myosins for PCR Primer Design -- Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena -- Microscopy Applications -- Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function -- Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility -- Evaluation of Individual-Cell Motility -- Evaluation of Cell Morphology -- A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena -- Reagents for Studying Cytoskeleton Protein Function -- Jasplakinolide -- Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteins -- Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy -- Tat-Mediated Delivery of Antibodies into Cultured Cells -- Cytoskeleton Dynamics -- Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein -- Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics -- Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics -- Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function -- Chromatophores as Tools for the Study of Organelle Transport -- Centriole Duplication, Centrosome Maturation and Spindle Assembly in Lysates of Spisula solidissima Oocytes -- XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate Filament Interactions -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic Approaches for Studies of Cytoskeleton Protein Function -- A Yeast Two-Hybrid Approach for Probing Cytoskeletal Protein Interactions -- Manipulating Dynein Genes in Tetrahymena -- Functional Analysis of Cytoskeletal Components in the Developing Visual System of Drosophila.

بدون عنوان

0

متن يادداشت

Many recent advances in experimental instrumentation, reagents, and imaging technology have dramatically expanded the range of tools available for the study of the cytoskeleton. In Cytoskeleton Methods and Protocols, Ray Gavin brings together an international panel of experienced researchers to detail the readily reproducible methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. Each protocol contains proven step-by-step instructions that enable both the novice and experienced researcher to achieve successful experimental results. The protocols utilize diverse model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional, often unpublished, information that can make the difference between success and failure. State-of-the-art and highly practical, Cytoskeleton Methods and Protocols makes available a diverse collection of powerful experimental systems and tools for successfully studying cytoskeleton structure and function.

شماره انبار

springerp

عنوان

Cytoskeleton methods and protocols.

شماره استاندارد بين المللي کتاب و موسيقي

9780896037717

موضوع مستند نشده

Cytoskeletal proteins, Laboratory manuals.

موضوع مستند نشده

Cytoskeleton, Laboratory manuals.

موضوع مستند نشده

Cytoskeletal Proteins.

موضوع مستند نشده

Cytoskeleton.

موضوع مستند نشده

Cytosquelette, Manuels de laboratoire.

موضوع مستند نشده

Protéines du cytosquelette, Manuels de laboratoire.

موضوع مستند نشده

Cytoskeletal proteins.

موضوع مستند نشده

Cytoskeleton.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Cell Biology.

موضوع مستند نشده

Wetenschappelijke technieken.

موضوع مستند نشده

PSF.

موضوع مستند نشده

SCI-- 017000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

Gavin, Ray H.

تاريخ عمليات

20201204223311.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y