عنوان

In situ detection of DNA damage :

شابک

1592591795

شابک

9781592591794

شابک اشتباه

0896039528

شابک اشتباه

9780896039520

شماره

b715213

عنوان اصلي

In situ detection of DNA damage :

نام عام مواد

[Book]

ساير اطلاعات عنواني

methods and protocols /

نام نخستين پديدآور

edited by Vladimir V. Didenko.

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

©2002.

نام خاص و کميت اثر

1 online resource (xvii, 313 pages, 4 unnumbered pages of plates) :

ساير جزييات

illustrations (some color)

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

v. 203

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

pt. 1. Labeling DNA breaks using terminal transferase (TUNEL assay) -- Labeling DNA damage with terminal transferase: applicability, specificity, and limitations / P. Roy Walker -- TUNEL assay: an overview of techniques / Deryk T. Loo -- Electron microscopic detection of DNA damage labeled by TUNEL / Antonio Migheli -- Quanitative differentiation of both free 3' OH and 5' OH DNA ends using terminal transferase-based labeling combined with transmission electron microscopy / Yoshinori Otsuki -- Determination of three-dimensional distribution of apoptotic DNA damage by combination of TUNEL and quick-freezing and deep-etching techniques / Shinichi Ohno -- In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser-scanning cytometry / Zbigniew Darzynkiewicz -- pt. 2. Labeling DNA breaks using DNA polymerase I or its Klenow fragment -- DNA damage detection using DNA polymersase I or its Klenow fragment: applicability, specificity, limitations / Jan Hein van Dierendonck -- Labeling DNA breaks in situ by Klenow enzyme / Katherine A. Wood -- In situ nick translation at the electron microscopic level / Marc Thiry -- pt. 3. Labeling DNA breaks using ligase -- In situ DNA ligation as a method for labeling apoptotic cells in tissue sections: an overview / Peter J. Hornsby -- Detection of specific double-strand DNA breaks and apoptosis in situ using T4 DNA ligase / Vladimir V. Didenko -- In situ detection of double-strand DNA breaks with terminal 5'OH groups / Vladimir V. Didenko -- pt. 4. Detection of DNA breaks in agarose trapped cells: comet assay and related techniques -- The comet assay: principles, applications, and limitations / Andrew R. Collins -- The comet assay: an overview of techniques / Peggy L. Olive -- Ultrasensitive detection of DNA damage by the combination of the comet and TUNEL assays / Andrei L. Kindzelskii -- Application of FISH to detect DNA damage: DNA breakage detection-FISH (DBD-FISH) / José Luis Fernández -- pt. 5. Detection of modified bases and AP sites in DNA -- Simultaneous in situ detection of DNA fragmentation and RNA/DNA oxidative damage using TUNEL assay and immunohistochemical labeling for 8-hydroxy-2'-deoxyguanosine (8-OhdG) / Alexander E. Kalyuzhny -- The in situ detection of apurinic/apyrimidinic sites and DNA breaks bearing extension blocking termini / Philip K. Liu -- pt. 6. Indirect and general markers of DNA damage -- Markers of poly (ADP-ribose) polymerase activity as correlates of DNA damage / Yinong Zhou -- Ultrasound imaging of apoptosis: DNA-damage effects visualized / Gregory J. Czarnota -- p53 induction as an indicator of DNA damage / Galina Selivanova -- -Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA) / Zbigniew Darzynkiewicz.

بدون عنوان

0

متن يادداشت

Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.

عنوان

In situ detection of DNA damage.

شماره استاندارد بين المللي کتاب و موسيقي

9780896039520

عنوان

Springer Protocols

موضوع مستند نشده

DNA damage-- Research-- Methodology.

موضوع مستند نشده

Clinical Laboratory Techniques.

موضوع مستند نشده

DNA-- analysis.

موضوع مستند نشده

DNA Damage.

موضوع مستند نشده

ADN-- Lésions-- Recherche-- Méthodologie.

موضوع مستند نشده

Beschadigingen.

موضوع مستند نشده

Detectie.

موضوع مستند نشده

DNA.

موضوع مستند نشده

SCIENCE-- Life Sciences-- Genetics & Genomics.

موضوع مستند نشده

Wetenschappelijke technieken.

موضوع مستند نشده

MFN

موضوع مستند نشده

SCI-- 029000

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

نشانه اثر

نشانه اثر

مستند نام اشخاص تاييد نشده

Didenko, Vladimir V.

تاريخ عمليات

20201208053014.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y