عنوان

Biophysics and the challenges of emerging threats /

شابک

9048123682

شابک

9789048123681

شابک اشتباه

9048123666

شابک اشتباه

9048123674

شابک اشتباه

9789048123667

شابک اشتباه

9789048123674

شماره

b710296

عنوان اصلي

Biophysics and the challenges of emerging threats /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Joseph D. Puglisi.

محل نشرو پخش و غیره

Dordrecht :

نام ناشر، پخش کننده و غيره

Springer,

تاریخ نشرو بخش و غیره

©2009.

نام خاص و کميت اثر

1 online resource (vii, 179 pages) :

ساير جزييات

illustrations

عنوان فروست

NATO science for peace and security series. Series B, physics and biophysics

متن يادداشت

Selected conference papers.

متن يادداشت

Includes bibliographical references and index.

متن يادداشت

A simple model for protein folding / Eric R. Henry and William A. Eaton -- Complementarity of hydrophobic/hydrophilic properties in protein-Ligand complexes: a new tool to improve docking results / Timothy V. Pyrkov [and others] -- Structures of Cvnh family lectins / Angela M. Gronenborn -- Biophysical approaches to study DNA base flipping / Saulius Klimasauskas, Zita Liutkeviciute and Dalia Daujotyte -- The diversity of nuclear magnetic resonance spectroscopy / Corey W. Liu [and others] -- Improved dye stability in single-molecule fluorescence experiments / Colin Echeverria Aitken, R. Andrew Marshall and Joseph D. Pugi -- The evaluation of isotope editing and filtering for protein-Ligand interaction elucidation by Nmr / Ian M. Robertson, Leo Spyracopoulos and Brian D. Sykes -- Ribosome: an ancient cellular nano-machine for genetic code translation / Ada Yonath.

بدون عنوان

0

متن يادداشت

Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1-3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4-6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11-13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16-18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. Oxidized dyes are no longer fluor- cent; oxidative damage impairs the folding and function of biomolecules. In the presence of saturating dissolved O, blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid. Enzymatic O -scavenging systems are commonly employed to ameliorate dye instability. 2 Small molecules are often employed to suppress blinking at low O levels.

منبع سفارش / آدرس اشتراک

Springer

شماره انبار

978-90-481-2367-4

عنوان

Biophysics and the challenges of emerging threats.

شماره استاندارد بين المللي کتاب و موسيقي

9048123666

موضوع مستند نشده

Biophysics, Congresses.

موضوع مستند نشده

Bioterrorism, Congresses.

موضوع مستند نشده

Biophysics.

موضوع مستند نشده

Biophysics.

موضوع مستند نشده

Bioterrorism.

موضوع مستند نشده

Bioterrorism.

موضوع مستند نشده

Physique.

موضوع مستند نشده

PSA.

موضوع مستند نشده

SCI086000.

شماره

ويراست

شماره رده

نشانه اثر

شماره رده

شماره رده

شماره رده

شماره رده

نشانه اثر

کد سيستم

کد سيستم

مستند نام اشخاص تاييد نشده

Puglisi, Joseph D.

مستند نام تنالگان تاييد نشده

NATO Advanced Study Institute on Biophysics and the Challenges of Emerging Threats(2007 :, Erice, Italy)

تاريخ عمليات

20201208010311.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y