عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
LA9s42p142
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
The Dynamics of Prophages/Phages FP1 and FP2 of 'Candidatus Liberibacter asiaticus' in Response to Stress Conditions
نام عام مواد
[Article]
نام نخستين پديدآور
Ding, Fang; Zhang, S.; Duan, Yong-ping
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
'Candidatus Liberibacter asiaticus' (Las), the prevalent bacterial pathogen associated with citrus huanglongbing (HLB), harbors at least two prophages, named FP1 and FP2. Due to the fastidious nature of Las, little is known about the prophage's response to stress conditions. In this study, we used real time PCR to investigate the potential conversion of the FP1 and FP2 prophages under stress conditions by comparing the 16S rDNA copy number in HLB-affected periwinkle and citrus. When HLB-affected periwinkle was exposed to heat stress for 4.0 hours, more FP1 and FP2 phage particles were released at 42℃ and 45℃ than at 37℃. A temperature increase from 23℃ to 37℃ caused the relative copy numbers of FP1 and FP2 to increase six folds, while a shift from 23℃ to 42℃ or 45℃ caused the relative copy numbers of FP1 and FP2 to increase between 7.5 and 15-folds compared to the initial samples. Meanwhile, similar results were found when HLB-affected citrus scions were treated with tetracycline at concentrations of 500 ppm to 2000 ppm by soaking for three days. When treated with tetracycline for 7 to 9 hours, the relative copy numbers of FP1 and FP2 reached their highest levels with an increase of 6 to 11.1-folds compared to the initial samples. The results indicate that stress causes the prophages in Las to convert from the lysogenic to lytic cycle. Furthermore, transmission electron microscopy (TEM) provided direct evidence that an upward temperature shift is accompanied by the lysogenic to lytic conversion. This conversion from the lysogenic to lytic cycle may have applications in terms of modulating HLB populations in naturally occurring infections. The study gives new insight into the interaction of prophages and HLB, which may play a potentially important role in the control of HLB.
مجموعه
تاريخ نشر
2014
عنوان
Journal of Citrus Pathology
شماره جلد
1
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
عنصر شناسه اي
Ding, Fang; Zhang, S.; Duan, Yong-ping
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب اطلاعات رکورد کتابشناسی
نوع ماده
[Article]
کد کاربرگه
275578
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
