عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
LA1mh3233b
عنوان و نام پديدآور
عنوان اصلي
Histological and Functional Benefit Following Transplantation of Motor Neuron Progenitors to the Injured Rat Spinal Cord
نام عام مواد
[Article]
نام نخستين پديدآور
Rossi, Sharyn L.; Nistor, Gabriel; Wyatt, Tanya; Yin, Hong Zhen; Poole, Aleksandra J.; Weiss, John H.; Gardener, Matthew J.; Dijkstra, Sipke; Fischer, David F.; Keirstead, Hans S.
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
BackgroundMotor neuron loss is characteristic of cervical spinal cord injury (SCI) and contributes to functional deficit.Methodology/Principal FindingsIn order to investigate the amenability of the injured adult spinal cord to motor neuron differentiation, we transplanted spinal cord injured animals with a high purity population of human motor neuron progenitors (hMNP) derived from human embryonic stem cells (hESCs). In vitro, hMNPs displayed characteristic motor neuron-specific markers, a typical electrophysiological profile, functionally innervated human or rodent muscle, and secreted physiologically active growth factors that caused neurite branching and neuronal survival. hMNP transplantation into cervical SCI sites in adult rats resulted in suppression of intracellular signaling pathways associated with SCI pathogenesis, which correlated with greater endogenous neuronal survival and neurite branching. These neurotrophic effects were accompanied by significantly enhanced performance on all parameters of the balance beam task, as compared to controls. Interestingly, hMNP transplantation resulted in survival, differentiation, and site-specific integration of hMNPs distal to the SCI site within ventral horns, but hMNPs near the SCI site reverted to a neuronal progenitor state, suggesting an environmental deficiency for neuronal maturation associated with SCI.Conclusions/SignificanceThese findings underscore the barriers imposed on neuronal differentiation of transplanted cells by the gliogenic nature of the injured spinal cord, and the physiological relevance of transplant-derived neurotrophic support to functional recovery.
مجموعه
تاريخ نشر
2010
عنوان
UC Irvine
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب اطلاعات رکورد کتابشناسی
نوع ماده
[Article]
کد کاربرگه
275578
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
