عنوان

Experience-dependent neuroplasticity of the developing hypothalamus:

شماره

LA4fk8j3pd

عنوان اصلي

Experience-dependent neuroplasticity of the developing hypothalamus:

نام عام مواد

[Article]

نام نخستين پديدآور

Ciernia, Annie VogelLaufer, BenjaminDunaway, KeithMordaunt, CharlesCoulson, RochelleTotah, TheresaStolzenberg, DanielleFrahm, JaimeSingh-Taylor, AkankshaBaram, TallieLaSalle, JanineYasui, Dag

عنوان اصلي به قلم نويسنده ديگر

integrative epigenomic approaches

متن يادداشت

Abstract Background Maternal care during early-life plays a crucial role in the sculpting of the mammalian brain. Augmented maternal care during the first postnatal week promotes life-long stress resilience and improved memory compared with the outcome of routine rearing conditions. Recent evidence suggests that this potent phenotypic change commences with altered synaptic connectivity of stress sensitive hypothalamic neurons. However, the epigenomic basis of the long-lived consequences is not well understood. Methods Here, we employed whole-genome bisulfite sequencing (WGBS), RNA-sequencing (RNA-seq), and a multiplex microRNA (miRNA) assay to examine the effects of augmented maternal care on DNA cytosine methylation, gene expression, and miRNA expression. Results A significant decrease in global DNA methylation was observed in offspring hypothalamus following a week of augmented maternal care, corresponding to differential methylation and expression of thousands of genes. Differentially methylated and expressed genes were enriched for functions in neurotransmission, neurodevelopment, protein synthesis, and oxidative phosphorylation, as well as known stress response genes. Twenty prioritized genes with three lines of evidence (methylation, expression, and altered miRNA target) were identified as highly relevant to the stress resiliency phenotype. Conclusions This combined unbiased approach enabled the discovery of novel genes and gene pathways that advance our understanding of the central epigenomic mechanisms underlying the profound effects of maternal care on the developing brain.

تاريخ نشر

2017

عنوان

UC Davis

نام الکترونيکي

نوع ماده

[Article]

کد کاربرگه

277944

سطح دسترسي

a

تكميل شده

Y