عنوان

Engineering a robust DNA circuit for the direct detection of biomolecular interactions /

شابک

9789811321870

شابک

9789811321887

شابک

9789811321894

شابک

9811321876

شابک

9811321884

شابک

9811321892

شابک اشتباه

9789811321870

عنوان اصلي

Engineering a robust DNA circuit for the direct detection of biomolecular interactions /

نام عام مواد

[Book]

نام نخستين پديدآور

Ang Yan Shan.

محل نشرو پخش و غیره

Singapore :

نام ناشر، پخش کننده و غيره

Springer,

تاریخ نشرو بخش و غیره

2018.

نام خاص و کميت اثر

1 online resource (xlv, 188 pages) :

ساير جزييات

illustrations (some color)

عنوان فروست

Springer theses,

شاپا ي ISSN فروست

2190-5053

متن يادداشت

"Doctoral thesis accepted by National University of Singapore, Singapore."

متن يادداشت

Introduction -- Literature review -- Materials and methods -- Modular framework for engineering a self-contained DNA circuit -- Designing hybridization chain reaction monomers for robust signal amplification -- Design concepts in association toehold for robust signal transduction -- DNA split proximity circuit as a general platform for interrogating biomolecular events -- DNA split proximity circuit for visualizing cell surface receptor clustering -- a case study using human epidermal growth factor receptor family -- Conclusion and future outlooks.

بدون عنوان

0

متن يادداشت

This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The assembly of the localized circuit is dynamically driven by the recognition of biomolecules - a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally - which will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors.

شماره استاندارد بين المللي کتاب و موسيقي

9789811321870

شماره استاندارد بين المللي کتاب و موسيقي

9789811321894

موضوع مستند نشده

DNA.

موضوع مستند نشده

Nanobiotechnology.

موضوع مستند نشده

DNA.

موضوع مستند نشده

Nanobiotechnology.

موضوع مستند نشده

TC

موضوع مستند نشده

TC

موضوع مستند نشده

TEC009010

شماره

ويراست

شماره رده

مستند نام اشخاص تاييد نشده

Yan Shan, Ang

تاريخ عمليات

20200823230131.0

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y