عنوان

Isolation and characterisation of novel glycosaminoglycan-like polysaccharides derived from marine molluscs with antiproliferative activity

شماره

TLets781266

عنوان اصلي

Isolation and characterisation of novel glycosaminoglycan-like polysaccharides derived from marine molluscs with antiproliferative activity

نام عام مواد

[Thesis]

نام نخستين پديدآور

Aldairi, A. F.

نام ناشر، پخش کننده و غيره

University of Salford

تاریخ نشرو بخش و غیره

2018

جزئيات پايان نامه و نوع درجه آن

Thesis (Ph.D.)

امتياز متن

2018

متن يادداشت

Although various anticancer therapeutic tools have been launched, such as immunotherapy and targeted therapy, newly developed drugs with antiproliferative/cytotoxic effects are still in demand; hence, newly discovered anticancer drugs with an improved pharmacological profile are needed to overcome drug resistance of cancer cells. This study focused on identifying and characterising novel polysaccharides isolated from the common cockle (Cerastoderma edule) with antiproliferative activity, using the cetylpyridinium chloride precipitation method. Marine polysaccharides have exhibited in-vitro potent antiproliferative activity, leading to induced apoptotic cellular death in three cancer cell lines, which are chronic myelogenous leukaemia, relapsed acute lymphoblastic leukaemia and mesothelioma of the pleural cavity with asbestos exposure. Structural analysis has confirmed the presence of glycosaminoglycan-like polysaccharides in cockle extracts with potent antiproliferative activity, which are susceptible to three classes of heparinase enzymes, which has not been seen previously, but not to chondroitinase enzymes. Cockle polysaccharide purification using anion-exchange chromatography has generated six peaks (1-6); however, only fraction 5 exhibited antiproliferative activity, which was determined to be susceptible to heparinase enzymes. Surprisingly, some sequence of resistance to heparinase was observed within cockle polysaccharide chains; therefore, a new type of marine-derived heparan sulphate/heparin-like polysaccharide with potent anticancer properties was suggested, as none of the mammalian glycosaminoglycans exhibited antiproliferative activity on cancer cell lines. Overall, the final structural characterisation did confirm the presence of fucosylated polysaccharide, N- and O-linked glycans, in addition to HS-like structures. These various chains made creating the final judgment about cockle polysaccharide structural elucidation very challenging because of sample complexity.

مستند نام اشخاص تاييد نشده

Aldairi, A. F.

مستند نام تنالگان تاييد نشده

University of Salford

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y