عنوان

Studies on gene expression in a pathogenic bacterium

شماره

TLets537288

عنوان اصلي

Studies on gene expression in a pathogenic bacterium

نام عام مواد

[Thesis]

نام نخستين پديدآور

Islam, Shahidul Md.

نام ناشر، پخش کننده و غيره

University of Birmingham

تاریخ نشرو بخش و غیره

2011

جزئيات پايان نامه و نوع درجه آن

Thesis (Ph.D.)

امتياز متن

2011

متن يادداشت

The activity of the major pathogenicity determinant of enterohaemorrhagic Escherichia coli is primarily coordinated by expression of the LEE1 operon which is part of the locus of enterocyte effacement (LEE). The LEE1 operon regulatory region has been dissected. LEE-encoded transcription factor, GrlA, activates the LEE1 P1 promoter by binding to a target located within the 18 base pair spacer between the promoter 10 and 35 elements. Shortening this spacer to 17 base pairs increases P1 promoter activity and short-circuits GrlA dependent activation, suggesting that GrlA functions like MerR family transcription activators. It was also found that the LEE1 P1 promoter is overlapped by a cryptic promoter, designated P1A. A single base substitution in the P1 consensus -35 element unmasks P1A promoter activity. In contrast, P1A activity is much less when P1 is inactivated by a mutation in its -10 hexamer element. Hence, even when P1 is inactive, the consensus -35 element sequesters RNA polymerase and prevents its access to the P1A promoter. The LEE1 leader sequence also contains a mini-gene that encodes a dipeptide. Genetic studies showed that expression of this mini-gene is important for optimal expression of downstream genes.

موضوع مستند نشده

QR Microbiology

مستند نام اشخاص تاييد نشده

Islam, Shahidul Md.

مستند نام تنالگان تاييد نشده

University of Birmingham

نام الکترونيکي

فرمت انتشار

p

نوع ماده

[Thesis]

کد کاربرگه

276903

سطح دسترسي

a

تكميل شده

Y