عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
شماره کتابشناسی ملی
شماره
TL48885
زبان اثر
زبان متن نوشتاري يا گفتاري و مانند آن
انگلیسی
عنوان و نام پديدآور
عنوان اصلي
A Systems-Level Investigation of the Metabolism of Dehalococcoides mccartyi and the Associated Microbial Community
نام عام مواد
[Thesis]
نام نخستين پديدآور
Mohammad Ahsanul Islam
نام ساير پديدآوران
Mahadevan, Radhakrishnan; Edwards, Elizabeth A.
وضعیت نشر و پخش و غیره
نام ناشر، پخش کننده و غيره
University of Toronto (Canada)
تاریخ نشرو بخش و غیره
2014
مشخصات ظاهری
نام خاص و کميت اثر
261
يادداشت کلی
متن يادداشت
Committee members: Master, Emma R.; Provart, Nicholas J.; Yakunin, Alexander F.; de-crecy Laggard, Valerie
یادداشتهای مربوط به نشر، بخش و غیره
متن يادداشت
Place of publication: United States, Ann Arbor; ISBN=978-1-339-87034-2
یادداشتهای مربوط به پایان نامه ها
جزئيات پايان نامه و نوع درجه آن
Ph.D.
نظم درجات
Chemical Engineering and Applied Chemistry
کسي که مدرک را اعطا کرده
University of Toronto (Canada)
امتياز متن
2014
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Dehalococcoides mccartyi are a group of strictly anaerobic bacteria important for the detoxification of man-made chloro-organic solvents, most of which are ubiquitous, persistent, and often carcinogenic ground water pollutants. These bacteria exclusively conserve energy for growth from a pollutant detoxification reaction through a novel metabolic process termed organohalide respiration. However, this energy harnessing process is not well elucidated at the level of D. mccartyi metabolism. Also, the underlying reasons behind their robust and rapid growth in mixed consortia as compared to their slow and inefficient growth in pure isolates are unknown. To obtain better insight on D. mccartyi physiology and metabolism, a detailed pan-genome-scale constraint-based mathematical model of metabolism was developed. The model highlighted the energy-starved nature of these bacteria, which probably is linked to their slow growth in isolates. The model also provided a useful framework for subsequent analysis and visualization of high-throughput transcriptomic data of D. mccartyi . Apart from confirming expression of the majority genes of these bacteria, this analysis helped review the annotations of metabolic genes. Revised annotations of two such metabolic genes - NADP+-isocitrate dehydrogenase and phosphomannose isomerase - were then experimentally verified. Finally, growth experiments were performed with a D. mccartyi -containing anaerobic mixed enrichment culture to explore the effects of exogenous vitamin omission from the growth medium on D. mccartyi and the associated microbial community. The experiments showed how nutritional requirements of these bacteria changed the composition and dynamics of their associated microbial community. Overall, a systems-level approach was used in this research to obtain a fundamental and critical understanding of the metabolism and physiology of D. mccartyi in isolates, as well as in microbial communities they naturally inhabit. The results presented in this thesis, therefore, will help design effective strategies for future bioremediation efforts by D. mccartyi.
موضوع (اسم عام یاعبارت اسمی عام)
موضوع مستند نشده
Biochemistry; Biomedical engineering; Bioinformatics; Environmental engineering
اصطلاحهای موضوعی کنترل نشده
اصطلاح موضوعی
Pure sciences;Biological sciences;Applied sciences;Bioremediation;Dehalococcoides mccartyi;Genome-scale model;Metabolic network
نام شخص به منزله سر شناسه - (مسئولیت معنوی درجه اول )
مستند نام اشخاص تاييد نشده
Cobb, Gavin
نام شخص - ( مسئولیت معنوی درجه دوم )
مستند نام اشخاص تاييد نشده
Mahadevan, Radhakrishnan; Edwards, Elizabeth A.
شناسه افزوده (تنالگان)
تقسيم فرعي
Chemical Engineering and Applied Chemistry
مستند نام تنالگان تاييد نشده
University of Toronto (Canada)
شماره دستیابی
شماره بازیابی
1814774331; 10129056
دسترسی و محل الکترونیکی
نام الکترونيکي
مطالعه متن کتاب وضعیت انتشار
فرمت انتشار
p
اطلاعات رکورد کتابشناسی
نوع ماده
[Thesis]
کد کاربرگه
276903
اطلاعات دسترسی رکورد
سطح دسترسي
a
تكميل شده
Y
