عنوان

Yeast protocols /

شابک

1588294374 (alk. paper)

شابک

1592599583 (e-ISBN)

شابک

9781588294371 (alk. paper)

شابک

9781592599585 (e-ISBN)

شماره

b517387

عنوان اصلي

Yeast protocols /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Wei Xiao

وضعيت ويراست

2nd ed

وضعيت ويراست

2nd ed

محل نشرو پخش و غیره

Totowa, N.J. :

نام ناشر، پخش کننده و غيره

Humana Press,

تاریخ نشرو بخش و غیره

[2006]

تاریخ نشرو بخش و غیره

©2006

نام خاص و کميت اثر

xi, 392 pages :

ساير جزييات

illustrations ;

ابعاد

23 cm.

عنوان فروست

Methods in molecular biology ;

مشخصه جلد

313

متن يادداشت

Includes bibliographical references and index

متن يادداشت

Basic investigations in saccharomyces cerevisiae / Brendan P.G. Curran and Virginia Bugeja -- Isolation of nucleic acids / Michelle Hanna and Wei Xiao -- Purification of yeast peroxisomes / Ben Distal and Astrid Kragt -- Isolation of yeast plasma membranes / Barry Panaretou and Peter Piper -- Isolation of yeast mitochondria / Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott -- Extraction of yeast lipids / Roger Schneiter and Günther Daum -- Two-dimensional gel electrophoresis of total yeast proteins / Hélian Boucherie and Christelle Monribot-Espagne -- Pulsed field gel electrophoresis of budding yeast chromosomes / Laura Maringele and David Lydall -- Analysis of yeast lipids / Roger Schneiter and Günther Daum -- Yeast fluorescence microscopy / Jirí Hasek

متن يادداشت

Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe / Michelle A. Alting-Mees [and others] -- Yeast transformed by the LiAc/SS carrier DNA/PEG method / R. Daniel Gietz and Robin A. Woods -- Mutagenesis / Leslie Barbour, Michelle Hanna, and Wei Xiao -- Gene disruption in the budding yeast saccharomyces cerevisiae / Johannes H. Hegemann, Ulrich Güldener, and Gabriele J. Köhler -- Inducible degron and its application to creating conditional mutants / R. Jürgen Dohmen -- Synthetic lethal screen / Leslie Barbour and Wei Xiao -- Synthetic genetic array analysis in saccharomyces cerevisiae / Amy Hin Yan Tong and Charles Boone -- Two-dimensional agarose gel analysis of DNA replication intermediates / Alain T. Dandjinou [and others] -- Chromatin assembly in a crude fraction from yeast cells / Karen M. Robinson and Michael C. Schultz -- Chromatin immunoprecipitation to study protein/DNA interactions in budding yeast / Elena Ezhkova and William P. Tansey

متن يادداشت

Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning / Zhengjian Zhang and Joseph C. Reese -- Study of transcriptional regulation using a reporter gene assay / Yu Fu and Wei Xiao -- Assessing telomeric phenotypes / Catherine LeBel [and others] -- Controlled expression of recombinant genes and preparation of cell-free extracts in yeast / Zhigang Wang -- Production of heterologous proteins in yeast with the aid of the Hsp150-Carrier / Marja Makarow [and others] -- Yeast two-hybrid system screening / R. Daniel Gietz -- Import of precursor proteins into isolated yeast mitochondria / Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling

بدون عنوان

0

بدون عنوان

0

بدون عنوان

0

متن يادداشت

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms

متن يادداشت

Mode of access: World Wide Web

عنوان

Yeast protocols.

شماره استاندارد بين المللي کتاب و موسيقي

1588294374

عنوان

OhioLINK electronic book center

عنوان

SpringerLink

موضوع مستند نشده

Yeast fungi-- Biotechnology, Laboratory manuals.

موضوع مستند نشده

Yeast fungi, Laboratory manuals.

موضوع مستند نشده

Yeasts-- cytology.

موضوع مستند نشده

Yeasts-- isolation & purification.

موضوع مستند نشده

Yeasts-- physiology.

مستند نام اشخاص تاييد نشده

Xiao, Wei,1957 April 10-

مستند نام تنالگان تاييد نشده

Ohio Library and Information Network.

تاريخ عمليات

20081117041421.0

قواعد فهرست نويسي ( بخش توصيفي )

rda

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y