عنوان

Modern techniques for pathogen detection /

شابک

3527335161

شابک

3527687971

شابک

352768798X

شابک

3527687998

شابک

3527688005

شابک

9783527335169

شابک

9783527687978

شابک

9783527687985

شابک

9783527687992

شابک

9783527688005

شابک اشتباه

9783527335169

شماره

dltt

عنوان اصلي

Modern techniques for pathogen detection /

نام عام مواد

[Book]

نام نخستين پديدآور

edited by Jürgen Popp and Michael Bauer

نام خاص و کميت اثر

1 online resource

متن يادداشت

Includes index

متن يادداشت

Includes bibliographical references at the end of each chapters and index

متن يادداشت

Unmet Medical Needs in Life-Threatening infections - Caring for the Critically Ill / Michael Bauer, Andreas Kortgen, Mervyn Singer -- Life Threatening Infections and Sepsis - Defining the Problem -- Sepsis as a "Hidden Healthcare Disaster" -- Microorganisms and Types of Infection Triggering Sepsis -- Emerging Problems Related to Resistance in Bacterial Infections -- The Role of Fungi and Viruses -- The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis -- Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection -- Beyond Infection - Profiling the Immune Response of the Septic Host -- Host Factors Contributing to Pathogenesis of Sepsis -- References -- Identification Methods - An Overview / Oliwia Makarewicz, Claudia Stein, Wolfgang Pfister, Bettina Löffler, Mathias Pletz -- Taxonomy of Pathogenic Organisms -- Microscopic Methods -- Stains -- Autofluoresccnce -- Immunofluorescence -- In Situ Hybridization -- Dark-Field Microscopy -- Culture-Based Methods -- Blood Culture -- Automated Differentiation Systems -- API System -- Selective Agar -- Susceptibility Testing -- Other Methods -- Nucleic Acid-Based Techniques -- PCR -- Real-Time PCR -- Multiplex-PCR -- RAPD and rep-PCR -- Microaarrays -- RFLP -- Sequencing -- Ribosomal RNA Genes -- MLST -- NG Sequencing -- Serology -- Antigen-Antibody-Based Methods -- Agglutination -- Immunodiffusion -- Quantitative Immunoassays -- Automated Immunoassays -- Applications of Serological Test -- Types of Human Antibodies and What They Indicate? -- Validity of Antibodies against Opportunistic Species -- Specificity of Fungal Antigens -- Specific Urine Antigen Tests -- Biomarkers -- Conclusions and Perspectives -- References -- Nucleic Acid Amplification Techniques / Marc Lehmann, Roland P.H. Schmitz -- Introduction -- The Basic PCR Protocol -- Primer Design -- Specific versus Broad-Range Primers and the Fate of False Targeting -- Modified End-Point PCR Protocols -- Non-PCR NAT: isothermal Amplification Protocols -- Quantitative PCR -- Quantification in qPCR -- Melting Curve Analysis (MCA) -- Controls, Probing, and General Aspects of Result Interpretation -- Strategies in Amplicon Verification -- (DNA) Microarray -- Flow Cytometry -- Mass Spectrometry -- PCR Inhibitors -- Preanalyses -- Sample Volume -- Cell Lysis -- Nucleic Acid Isolation and Preparation -- Fields of PCR Application -- Sequencing -- Pyrosequencing -- Typing and Epidemiology -- Pathogen Detection in Complex Clinical Samples -- General Aspects of Result Interpretation -- Sensitivity and Specificity -- NAT versus Culture -- The Fate of Antibiotic Resistances Detection -- Microbial Trace Detection in BSI, Ascites, and Synovial Fluids -- Grown Blood Culture Media as Samples -- Upcoming Routine Solutions - Dawn of Assay Automation -- Conclusion and Perspective -- Acknowledgment -- References -- DNA Microarrays for Pathogen Detection / Holger Schulze, Maya Rubtsova, Till T. Bachmann -- Introduction -- DNA Microarrays for the Detection of Bacterial Pathogens -- Major Bacterial Diseases -- Blood Stream Infections/Sepsis -- Pneumonia -- Infectious Diarrhea -- Antibiotic Resistance Detection -- Gram-Negative Bacteria -- Gram-Positive Bacteria -- Staphylococcus aureus -- Mycobacteria -- DNA Microarrays for Virus Diagnostics -- Human Papillomaviruses -- Commercial HPV Tests -- Respiratory Viruses -- Enteric Viruses -- Hepatitis Viruses -- DNA Microarrays for Detection of Fungal Pathogens -- DNA Microarrays for Parasite Diagnostics -- Malaria -- Waterborne Parasites -- Other Parasites -- Conclusion an Outlook -- References -- MALDI-ToF / Stefan Zimmermann -- Introduction -- MALDI-ToF Technology -- Bacterial Identification Using Mass Spectrometry -- Culture-Independent Rapid Identification of Clinical Pathogens -- Antibiotic Susceptibility Testing Using Mass Spectrometry -- References -- IR and Raman Spectroscopy for Pathogen Detection / Ute Münchberg, Sandra Kloß, Dragana Kusic, Susann Meisel, Ralf Heinke, Stephan Stöckel, Petra Rösen, Jürgen Popp -- Introduction -- Bulk Analysis -- IR and NIR Absorption Spectroscopy -- Raman Spectroscopy with Excitation in the NIR Region -- Resonance Raman Spectroscopy -- Excitation with Visible Wavelengths -- UV-Resonance Raman Spectroscopy -- Surface-Enhanced Raman Spectroscopy -- The SERS Effect -- Analysis of Bulk Bacteria Samples with SERS -- Single-Cell Analyses -- Micro-Raman Spectroscopy with Excitation in the Visible or NIR Region -- Surface-Enhanced Raman Spectroscopy on Single Cells -- Conclusion and Outlook -- References -- FISH / Graeme N. Forrest, Jwan Mohammadi, Shahrzad Mohammadi -- Introduction -- FISH Techniques -- PNA FISH -- Original PNA FISH Platform -- PNA FISH Flow Cytometry -- Beacon-Based FISH (bbFISH) -- Clinical Implications of PNA FISH -- Gram-Positive Organisms -- Staphylococci -- Enterococci -- Gram-Negative Probes -- Candida -- Beta Hemolytic Streptococci -- Mycobacteria -- Helicobacter pylori -- Other Bacteria -- Parasites -- Summary and Outlook -- References -- Conclusions / Sibyll Pollok, Karina Weber, Michael Bauer, Jürgen Popp

بدون عنوان

0

شماره استاندارد بين المللي کتاب و موسيقي

9783527687978

موضوع مستند نشده

Pathogenic microorganisms-- Detection.

موضوع مستند نشده

TEC-- 010000

شماره

ويراست

شماره رده

مستند نام اشخاص تاييد نشده

Bauer, Michael

مستند نام اشخاص تاييد نشده

Popp, Jürgen

مستند نام تنالگان تاييد نشده

Ohio Library and Information Network.

تاريخ عمليات

20160603081227.7

قواعد فهرست نويسي ( بخش توصيفي )

pn

نام الکترونيکي

نوع ماده

[Book]

تكميل شده

Y