Membrane proteins -- engineering, purification and crystallization /
نام عام مواد
[Book]
نام نخستين پديدآور
Edited by Arun K. Shukla
مشخصات ظاهری
نام خاص و کميت اثر
xx, 624 pages, 18 unnumbered pages of plates :
ساير جزييات
illustrations (some color) ;
ابعاد
24 cm
فروست
عنوان فروست
Methods in enzymology,
مشخصه جلد
volume 557
شاپا ي ISSN فروست
0076-6879 ;
یادداشتهای مربوط به کتابنامه ، واژه نامه و نمایه های داخل اثر
متن يادداشت
Includes bibliographical references and indexes
یادداشتهای مربوط به مندرجات
متن يادداشت
Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes -- A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs -- Membrane preparation and solubilization -- Amphipathic agents for membrane protein study -- Quantification of detergent using colorimetric methods in membrane protein crystallography -- Solubilizatoin of G protein-coupled receptors: a convenient strategy to explore lipid-receptor interaction -- Overexpression, isolation, purification, and crystallization of NhaA -- Purification, refolding, and cyrstallization of the outer membrane protein OmpG from Excherichia coli -- Biophysical approaches to the study of LeuT, a prokaryotic homolog of neurotransmitter sodium symporters -- Generation of recombinant antibody fragments for membrane protein crystallization -- Phage display selections for affinity reagents to membrane proteins in nanodiscs --Antibody fragments for stabilization and crystallization of G protein-coupled receptors and their signaling complexes -- Conformational analysis of G protein-coupled receptor signaling by hydrogen/deuterium exchange mass spectometry -- EPR studies of gating mechanisms in ion channels -- Magic-angle-spinning solid-state NMR of membrane proteins -- Solution NMR structure determination of polytopic a-helical membrane proteins: a guide to spin label paramagnetic relaxation enhancement restraints -- Inducing two-dimensional crystallization of membrane proteins by dialysis for electron crystallography -- Crystallization of membrane proteins by vapor diffusion -- Bicelles coming of age: an empirical approach to bicelle crystallization -- Fluorescence recovery after photobleaching in lipidic cubic phase (LCP-FRAP): a precrystallization assay for membrane proteins -- Crystallization of proteins from crude bovine rod outer segments -- Crystallization of photosystem II for time-resolved structural studies using an x-ray free electron laser -- Major intrinsic protein superfamily: channels with unique structural features and diverse selectivity filters -- Comparative sequence-function analysis of the major facilitator superfamily: the "mix-and-match" method -- Elucidating ligand-modulated conformational landscape of GPCRs using cloud-computing approaches