edited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK.
وضعیت ویراست
وضعيت ويراست
Second edition.
وضعیت نشر و پخش و غیره
محل نشرو پخش و غیره
New York :
نام ناشر، پخش کننده و غيره
Humana Press,
تاریخ نشرو بخش و غیره
[2015]
فروست
عنوان فروست
Methods in molecular biology,
عنوان فروست
Springer protocols
مشخصه جلد
1300
شاپا ي ISSN فروست
1064-3745 ;
یادداشتهای مربوط به کتابنامه ، واژه نامه و نمایه های داخل اثر
متن يادداشت
Includes bibliographical references and index.
یادداشتهای مربوط به مندرجات
متن يادداشت
A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection and Sequencing of Okazaki Fragments In S. cerevisiae -- ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin -- Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors -- Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism -- Single-Molecule Observation of Prokaryotic DNA Replication -- Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.
بدون عنوان
0
یادداشتهای مربوط به خلاصه یا چکیده
متن يادداشت
Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.