عنوان

Yeast protocols

شابک

1493907980

عنوان اصلي

Yeast protocols

نام نخستين پديدآور

edited by Wei Xiao

وضعيت ويراست

Third edition.

محل نشرو پخش و غیره

New York

نام ناشر، پخش کننده و غيره

Springer Science]Business Media

تاریخ نشرو بخش و غیره

2014

نام خاص و کميت اثر

x, 313 pages : illustrations )some color(

عنوان فروست

Methods in Molecular Biology,1064-3745 ;1163

عنوان فروست

Springer protocols,1949-2448

عنوان فروست

Series: Methods in molecular biology )Clifton, N.J.( ;v. 1163,1064-3745

عنوان فروست

Series: Springer protocols )Series(,1949-2448

متن يادداشت

Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.

متن يادداشت

Basic investigations in Saccharomyces cerevisiae /Brendan P.G. Curran and Virginia Bugeja --Isolation of yeast nucleic acids /Michael Biss, Michelle D. Hanna, and Wei Xiao --Quantification of GFP signals by fluorescent microscopy and flow cytometry /Ting Wei and Heping Dai --Yeast transformation by the LiAc/SS carrier DNA/PEG method /R. Daniel Gietz --Targeted gene deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe /Johannes H. Hegemann ]and others[ --See & catch method for studying protein complexes in yeast cells : a technique unifying fluorescence microscopy and mass spectrometry /Changhui Deng and Andrew N. Krutchinsky --Yeast two-hybrid liquid screening /Elisa Donnard ]and others[ --Targeted mutagenesis of a specific gene in yeast /Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz --Fluorescence staining of mitochondria for morphology analysis in Saccharomyces cerevisiae /Kai Stefan Dimmer --Assays for autophagy I : the Cvt pathway and nonselective autophagy /Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie --Assays for autophagy II : mitochondrial autophagy /Tomotake Kanki and Koji Okamoto --Biochemically characterizing the subcellular localization of peroxisomal proteins by fractionation, protease protection, and carbonate extraction /Danielle Hagstrom and Changle Ma --Yeast survival and growth assays /Xin Xu, Amanda D. Lambrecht, and Wei Xiao --Spontaneous mutagenesis assay /Susan Blackwell, Michelle D. Hanna, and Wei Xiao --Mutational specificity analysis : assay for mutations in the yeast SUP4-o gene /Bernard A. Kunz --piggyBac transposon-based insertional mutagenesis for the fission yeast Schizosaccharomyces pombe /Jun Li and Li-Lin Du --Replicative and chronological life-span assays /Spike D.L. Postnikoff and Troy A.A. Harkness --Detection of protein arginine methylation in Saccharomyces cerevisiae /Christopher A. Jackson and Michael C. Yu --Detection of protein posttranslational modifications from whole-cell extracts in Saccharomyces cerevisiae /Lindsay G. Ball and Wei Xiao --Ligand engineering using yeast surface display /Benjamin J. Hackel --Screening combinatorial libraries of cyclic peptides using the yeast two-hybrid assay /Kris Barreto and C. Ronald Geyer.

متن يادداشت

Includes bibliographical reference and index.

عنصر شناسه ای

Yeast fungi^aBiotechnology--Laboratory manuals

عنصر شناسه ای

Yeast fungi^aLaboratory manuals

عنصر شناسه ای

Yeasts

عنصر شناسه ای

Laboratory Manuals

شماره

شماره رکورد غير از شماره رده بندي

شماره رده

شماره رکورد رده بندي

نشانه اثر

شماره رکورد غير از شماره رده بندي

عنصر شناسه اي

Xiao, Wei

تقسيم تاريخي

1957 April 10

نوع ماده

English Book