عنوان

PCR primer design

شابک

9781493923649hardback : acid-free paper

عنوان اصلي

PCR primer design

نام نخستين پديدآور

edited by Chhandak Basu, Department of Biology, California State University, Northridge, Los Angeles, CA, USA

وضعيت ويراست

Second edition.

محل نشرو پخش و غیره

New York

نام ناشر، پخش کننده و غيره

Springer

نام خاص و کميت اثر

xii, 216 pages : illustrations )some color( ; 27 cm.

عنوان فروست

Methods in molecular biology,1064-3745 ;1275

عنوان فروست

Springer protocols,1949-2448

عنوان فروست

Series: Methods in molecular biology )Clifton, N.J.( ;1275,1064-3745

عنوان فروست

Series: Springer protocols )Series(,1949-2448

متن يادداشت

"This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." --Provided by publisher.

متن يادداشت

Fast masking of repeated primer binding sites in eukaryotic genomes / Reidar Andreson, Lauris Kaplinksi, and Maido Remm -- Primer design for PCR reactions in forensic biology / Kelly M. Elkins -- Design of primers and probes for quantitative real-time PCR methods / Alicia Rodraiguez, Mar Rodraiguez, Juan J. Caordoba, and Maraia J. Andrade -- Large-scale neucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus / Alexander Nagy, Tomaaes Jierinec, Lenka eCernaikovaa, Helena Jierincovaa, and Martina Havlaieckovaa -- Low-concentration initiator primers improve the amplification of gene targets with high sequence variability / Kenneth E. Pierce and Lawrence J. Wangh -- Multiplex PCR primer design for simultaneousu detection of multiple pathogens / Wenchao Yan -- Degenerate primer design for highly variable genomes / Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell -- Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection / Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu -- MultiPLX: automatic grouping and evaluation of PCR primers / Lauris Kaplinski and Maido Remm -- In silico PCR primer designing and validation / Anil Kumar and Nikita Chrodia -- Primer design using Primer Expressش for SYBR green-based quantitative PCR / Amarjeet Singh and Girdhar K. Pandey -- Designing primers for SNaPshot technique / Greciane Gaburro Paneto and Francisco de Paula Careta -- Rapid and simple method of qPCR primer design / Brenda Thornton and Chhandak Basu -- PRIMEGENSw3: a web-based tool for high-throughput primer and probe design / Garima Kushwaha, Gyan Prakash Srivastava, and Dong Xu -- Selecting specific PCR primers with MFEprimer / Wubin Qu and Chenggang Zhang.

متن يادداشت

Includes bibliographical references and index.

عنصر شناسه ای

Polymerase chain reaction

عنصر شناسه ای

Gene amplification

عنصر شناسه ای

Gene amplification.^afast--)OCoLC(fst00939610

عنصر شناسه ای

Polymerase chain reaction.^afast--)OCoLC(fst01070553

شماره رده

شماره رکورد رده بندي

نشانه اثر

شماره رکورد غير از شماره رده بندي

عنصر شناسه اي

Basu, Chhandak

نوع ماده

English Book