عنوان

Practical biotransformations: a beginner's guide

.Language of Text, Soundtrack etc

English

Title Proper

Practical biotransformations: a beginner's guide

Place of Publication, Distribution, etc.

Chichester, West Sussex

Name of Publisher, Distributor, etc.

Wiley

Date of Publication, Distribution, etc.

c2009

Specific Material Designation and Extent of Item

xii, 331 p.: ill

Other Title Information

Postgraduate chemistry series

Text of Note

Includes bibliographical references and index

Text of Note

Gideon Grogan

Text of Note

1

Text of Note

Biotransformations, microbes and enzymes -- Introduction -- Biotransformations -- Microorganisms -- Organism nomenclature -- Enzymes -- Types of enzymatic reactions -- Enzymatic cofactors -- Some basic characteristics of enzyme catalysis -- Types of biocatalyst biotransformations by whole cells or isolated enzymes -- Conclusion -- An overview of biocatalyst sources and web-based information -- Introduction -- Microbial culture collections -- Obtaining organisms from other research groups -- Selective enrichments -- Metagenomics -- Enzyme suppliers and biocatalyst development companies -- Genome mining for biocatalysts -- Obtaining amino acid and gene sequence information on biocatalysts -- Obtaining DNA templates for cloning -- Custom gene synthesis -- Other interesting web resources for biocatalysis -- Conclusion -- Setting up a laboratory for biotransformations -- Introduction -- Microbiological containment -- On containment issues and genetically-modified organisms -- Equipment for handling microorganisms -- Techniques and terms in microbiology sterility, asepsis and aseptic technique -- Disposal of viable microbial waste and disinfection of reusable equipment -- Equipment for enzymology and molecular biology -- General reagents and chemicals in a biotransformations laboratory -- Conclusion -- A beginner's guide to preparative whole-cell microbial biotransformations -- Introduction -- Storage, maintenance and growth of microorganisms -- General microbiological methods -- Examples of whole-cell biotransformations using bacteria -- Biotransformation by filamentous fungi and yeasts -- Whole-cell biotransformations by recombinant strains of E. coli -- Conclusion -- A beginner's guide to biotransformations by commercially available isolated enzymes -- Introduction -- Lipases -- Hydrolytic reactions using lipases -- Using lipases for acylation reactions -- Other hydrolases -- Commercially available coenzyme-dependent enzymes -- Carbon-carbon bond forming reactions -- Conclusion -- A beginner's guide to the isolation and analysis and use of home-grown enzyme biocatalysts -- Introduction -- Cell growth and harvesting -- Cell disruption -- A typical procedure for making a cell extract from a recombinant strain of E. coli -- Purification of enzymes - a brief guide -- Techniques for protein purification -- Isolation of recombinant enzymes using histidine tags -- Estimation of protein

Entry Element

، Biosynthesis

Entry Element

، Enzymes - Biotechnology

Entry Element

، Microbial biotechnology

Entry Element

، Organic compounds - Synthesis

Number

Class number

Entry Element

Grogan, Gideon.

Relator Code

AU

TI

SE