عنوان
پدید آورنده
موضوع
رده
کتابخانه
محل استقرار
NATIONAL BIBLIOGRAPHY NUMBER
Country Code
IR
Number
۲۸۸پ
LANGUAGE OF THE ITEM
.Language of Text, Soundtrack etc
فارسی
COUNTRY OF PUBLICATION OR PRODUCTlON
Country of publication
IR
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
بررسی ژن گاماگلوبین در رده‌ی سلولی 6252K با بیان پایدارshRNA ی MBD2
General Material Designation
[پایان‌نامه]
Parallel Title Proper
Gamma globin induction in K562 cells stable knocking down MBD2
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
علوم بهزیستی و توانبخشی university of social welfare and rehabilitation))
Date of Publication, Distribution, etc.
، ۱۳۸۹
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
ل،۱۵۴ص.
GENERAL NOTES
Text of Note
پیوست
NOTES PERTAINING TO PUBLICATION, DISTRIBUTION, ETC.
Text of Note
چاپی
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
کارشناسی ارشد
Discipline of degree
ژنتیک genetic
Body granting the degree
علوم بهزیستی و توانبخشی university of social welfare and rehabilitation))
SUMMARY OR ABSTRACT
Text of Note
MBD2 (Methyl Binding Domain 2) has been shown to repress the human -globin gene in mouse models. We have previously shown that transient MBD2 knockdown (35 ) using siRNAs results in induction of -globin expression (1.75 fold) in K562 cell line. In order to achieve higher levels of knockdown and possibly -globin induction, a lentivirus system was used for shRNA delivery. First, functionality of the system was verified by using a lentiviral transfer vector containing a GFP expression-cassette. Next, five lentivirus transfer vectors containing MBD2 shRNAs were screened for target gene knockdown by qRT-PCR in HEK 293T transient transfection assays. The two most effective shRNAs gave >70 mRNA knockdown levels after normalization for transfection efficiency. These vectors were subsequently packaged and used to transduce K562 cells. Upon infection and antibiotic selection, a cell population stably expressing MBD2 shRNAs was obtained. The amount of MBD2 knockdown and - globin expression was assessed in these cells by relative qRT-PCR. In one cell line (shRNA-3), MBD2 was knocked down by 73 and -globin was induced 5-fold. In the other (shRNA-5), MBD2 was down-regulated by 51 and -globin increased by 11.75-fold. These results support those of the transient RNAi experiments. Moreover, these cell lines can now be used for delineating the mechanism of MBD2 repression
ba
PARALLEL TITLE PROPER
Parallel Title
Gamma globin induction in K562 cells stable knocking down MBD2
TOPICAL NAME USED AS SUBJECT
K562
(SUBJECT CATEGORY (Provisional
Subject Category Entry Element Text
۵۶۲K
Subject Category Entry Element Text
K562
PERSONAL NAME - PRIMARY RESPONSIBILITY
فراشی، سمانه
Farashi, Samaneh
PERSONAL NAME - SECONDARY RESPONSIBILITY
بنان، مهدی
نجم آبادی، حسین
ORIGINATING SOURCE
Country
ایران
Agency
دانشگاه علوم توان بخشی و سلامت اجتماعی
LOCATION AND CALL NUMBER
Call Number
۲۸۸پ
ELECTRONIC LOCATION AND ACCESS
Host name
288..pdf
Access number
محرمانه
Compression information
محرمانه
Date and Hour of Consultation and Access
288.pdf
Bits per second
3640247
Contact for access assistance
مرجع
Electronic Format Type
متن
File size
0
Record control number
۸۸۲پایا
DATA NOT CONVERTED FROM SOURCE FORMAT
Tag of the Source Format Field
محل نگهداری: دانشگاه علوم بهزیستی و توانبخشی
نمایه‌سازی قبلی
چاپی-الکترونیکی
TF
1
a
Y
