عنوان

تثبیت و میکروانکپسولاسیون باکتری پروبیوتیک لاکتوباسیلوس کازئی بر روی بستر زئولیت و ارزیابی زنده مانی آن در شرایط شبیه ساز معده - روده

Number

‭۱۱۸۸۸پ‬

.Language of Text, Soundtrack etc

per

Title Proper

تثبیت و میکروانکپسولاسیون باکتری پروبیوتیک لاکتوباسیلوس کازئی بر روی بستر زئولیت و ارزیابی زنده مانی آن در شرایط شبیه ساز معده - روده

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/آزاد محمدحسن زاده

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: ارس

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چاپی

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کارشناسی ارشد

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علوم و صنایع غذایی - تکنولوژی مواد غذایی

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‮‭۱۳۹۳/۱۱/۲۵‬

Body granting the degree

تبریز

Text of Note

The beneficial effects of probiotic microorganisms appear when they arrive in the intestinal medium, viable and in high enough number. The probiotic bacterial cells can be immobilized by microencapsulation and can be protected from the harsh conditions of low pH, bile salts and digestive enzymes that exist in GI tract, thus allowing safe delivery of the cells to the colon, where they will be released. The immobilization of microorganisms onto suitable materials as carriers is currently gaining much attention in various fields of biotechnology. The perfect carrier of microorganism should be inert, nontoxic, of porous structure, relatively cheap, easily available, environmental friendly. Among the inorganic carriers, zeolites have been shown as a good material for the immobilization of microorganisms. In this study Lactobacillus casei was immobilized by zeolite and encapsulated using calcium alginate-zeolit-starch, via extrogen technique, and were incubated in simulated gastric juice, simulated intestinal juice. The morphology and size of microcapsules were measured by scanning electron and optical microscopy. The results indicated that the survival of free cells, immobilized cells on zeolite and microencapsulated probiotic increased significantly in simulated gastro-intestinal condition (P < 0.05). Sodium Alginate-Zeolit-Starch coating played a significant role in the protection of probiotic bacteria in simulated gastro-intestinal condition. In the case of free L. casei, the initial mean survival count of 9/65 log CFU/mL reduced to zero after 180 min. Also, the initial mean survival count of immobilized onto zeolite, microencapsulation of Casei with algenate and encapsulation of the immobilizated Casei onto zeolit with alginate-zeolite-starch beads decreased to 3, 3/9 and 6/5 log CFU/mL respectively after 180 min. In general, this study indicated that immobilization and microencapsulation could successfully and significantly protect probiotic bacteria against adverse condition

محمدحسن زاده، آزاد

صوتی خیابانی، محمود، استاد راهنما

رضایی مکرم، رضا، استاد راهنما

صمدی کفیل، حسین، استاد مشاور

دیوبند، بهارک، استاد مشاور

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