NATIONAL BIBLIOGRAPHY NUMBER
Number
۱۹۷۷۹پ
LANGUAGE OF THE ITEM
.Language of Text, Soundtrack etc
per
TITLE AND STATEMENT OF RESPONSIBILITY
Title Proper
بررسی بیان ژنCCAT۲ - lncدر نمونه های سرطانی پستان منطقه‌ی شمال غرب ایران
Parallel Title Proper
Study expression of lncCCAT۲ in breast cancer patient in West-north Iran
First Statement of Responsibility
/سمیرا کاظم زاده
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
: علوم طبیعی
Date of Publication, Distribution, etc.
، ۱۳۹۵
Name of Manufacturer
، میرزائی
PHYSICAL DESCRIPTION
Specific Material Designation and Extent of Item
۷۹ص
NOTES PERTAINING TO PUBLICATION, DISTRIBUTION, ETC.
Text of Note
چاپی - الکترونیکی
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
کارشناسی ارشد
Discipline of degree
ژنتیک
Date of degree
۱۳۹۵/۰۶/۱۵
Body granting the degree
تبریز
SUMMARY OR ABSTRACT
Text of Note
امروزه سرطان پستان بیماری رایجی در سراسر دنیا است که افراد زیادی از داشتن آن رنج میبرند .از سوی دیگر روش های رایج تشخیصی و پیش آگهی تومورهای سرطانی پستان نتوانسته اند در امر شناسایی به موقع و کارساز درمانی مفید واقع شده باشند .lncCCAT۲یکی از ژنهای غیرکدشونده طویل می باشدکه در سال های اخیر نقش آنها در بروز سرطان گزارش شده است .در تحقیق حاضر بیان ژن lncCCAT۲ بعنوان مارکر احتمالی در تومورهای پستانی کارسینومای پستان مورد بررسی قرار گرفته است .بدین منظور بیان ژن CCAT۲ در نمونه های توموری وحاشیه به ظاهر سالم تومور جمع آوری شده از بیماران مورد نظر توسط تکنیک Real time PCR مطالعه گردید .نتایج بدست آمده نشان داده اند که بیان این ژن در بافت های تومور نسبت به بافت های حاشیه تومور کارسینومای پستان بیشتر است .همچنین در تومورهایی با درگیری بالاتر غدد لنفاوی بیش بیان این ژن را نشان می دهد، در مرحله ی پیشرفته ی بدخیمی تومور نیز بیش بیان این ژن را داریم .با بررسی های حاضر می توان ارتباط معنی داری بین پیشرفته بودن تومور با افزایش بیان این ژن را نشان داد .در مجموع می توان گفت که ژن CCAT۲ می تواند بعنوان فاکتور بالقوه ی تشخیصی در سرطان سینه بکار گرفته شود که نیازمند مطالعات بیشتر می باشد
Text of Note
Abstract: Introduction: Breast cancer (BC) is the common malignancy among women with approximately one million new case/year worldwide. Despite advances in early detection and progress in treatment have declined breast cancer mortality rate, BC continues to pose an enormous global healthcare problem. With the development of whole-genome sequencing technology, it was determined that less than 2 of the mammalian genome is in protein-encoded regions and remainder is in non-coding RNAs (ncRNAs). Long non-coding RNAs (lncRNA) are molecules with more than 200 nucleotides in length that could not be translated into proteins. LncRNAs have been considered to be new approaches of tumor biomarkers for early cancer diagnosis and prognosis. Colon cancer associated transcript 2 (CCAT2) is a type of newly discovered lncRNA with tumor marker potential. It has been shown that this gene is up-regulated in some types of cancers, such as colon, gastric and hepatocellular carcinomas. CCAT2 is located in 8q24 genomic region. The authors suggest that CCAT2 might exert its biological function as an oncogene in several kinds of cancer, and it is overexpression in tissue cancer and promotes tumor growth, metastasis, and chromosomal instability, however the detailed function of CCAT2 in breast cancer was still uncertain. In the current study, we investigated the diagnostic and prognostic values of lncRNAs CCAT2 in BC tissues And expression level of this gene in clinical breast cancer and adjacent non-tumor tissues, as well as analyzed its association with overall survival and progression-free survival of breast cancer patients. We then examined the relationship between CCAT2 expression levels in tumor tissues and the clinical prognostic features of breast cancer patients. Methods: We evaluated expression of lnc-CCAT2 in normal breast tissue and BC tissue by real-time PCR and further analyzed CCAT2 expression in 35 sample with regard to clinical, pathological and other biological factors. Results: Ling et al first discovered CCAT2, a novel noncoding RNA mapping to 8q24, in 2013. They found that CCAT2 is highly overexpressed in microsatellite- stable colorectal cancer and promotes tumor growth, metastasis, and chromosomal instability. Redis et al showed that CCAT2 may represent a valuable predictive marker of clinical outcomes for a specific subgroup of breast cancer patients. We measured tissues CCAT2 level in BC patients, and healthy control subjects. The result revealed that compared to margin tissue. CCAT2 level is increased in the BC patients. CCAT2 level in BC is correlated with FIGO stage, lymph node metastasis and ROC curve analysis showed that tissues CCAT2 level accurately discriminates BC tissues from margin sample. Furthermore, CCAT2 level could also accurately discriminate early stage from higher stage. We identified in in vitro model the impact of CCAT2 expression and having the highest expression in advance stage and lymph node positive diseases. The result showed that the expression of this gene significantly over-express in tumor tissue compared to non-tumoral specimens. In conclusion, our data demonstrate that non-coding lnc-CCAT2 gene could be considered as a diagnostic tumoral specimens from non-tumoral margin
PARALLEL TITLE PROPER
Parallel Title
Study expression of lncCCAT۲ in breast cancer patient in West-north Iran
PERSONAL NAME - PRIMARY RESPONSIBILITY
کاظم زاده، سمیرا
kazemzadeh, samira
ELECTRONIC LOCATION AND ACCESS
Public note
سیاه و سفید
نمایه‌سازی قبلی
