رنگزدایی رنگزاهای آلی توسط ترکیب آنزیمهای پراکسیداتیو - اکسیداتیو
First Statement of Responsibility
/حمید فتاحیثانی
.PUBLICATION, DISTRIBUTION, ETC
Name of Publisher, Distributor, etc.
: پردیس دانشگاه تبریز
Date of Publication, Distribution, etc.
، ۱۳۹۵
NOTES PERTAINING TO PUBLICATION, DISTRIBUTION, ETC.
Text of Note
چاپی
DISSERTATION (THESIS) NOTE
Dissertation or thesis details and type of degree
کارشناسی ارشد
Discipline of degree
مهندسی شیمی
Date of degree
۱۳۹۵/۱۱/۲۰
Body granting the degree
تبریز
SUMMARY OR ABSTRACT
Text of Note
یکی از مشکلات اساسی زندگی امروزی آلودگیهای زیست محیطی است که عمده این آلودگیها از فرایندهای استخراج، تولید و مصرف مواد شیمیایی شامل حلالها، رنگها و حشره کشها ناشی میشوند . استفاده روز افزون از مواد و محصولات شیمیایی در زندگی مدرن نیز سرعت تولید این آلودگیها را بهطور چشمگیری افزایش داده است به گونه ای که تغییرات محسوسی در شرایط زیست محیطی به وجود آمده است .روشهای متنوعی جهت حذف این آلایندهها وجود دارد که از جمله آن میتوان به فرآیندهای بیولوژیکی اشاره کرد .فرآیندهای بیولوژیکی به دلیل مزایای فراوانی که دارند مورد توجه پژوهشگران حوزههای مختلفی قرار گرفتهاند .از جمله این مزایا میتوان به انجام واکنشها در دما و فشار معمولی، سازگاری بالا با محیط زیست و صرفه اقتصادی بالا اشاره نمود
Text of Note
The aim of this thesis, Effects Compositions manganese peroxidase (MnP) and glucose oxidase (GOx) enzymes produced by the fungus Phanerochaete chrysosporium in the removal of organic dyes and increase the duration of the presence of these enzymes is in and out of the culture medium. In the first part of the experiments, the effects of GOx addition on enzymatic activity of manganese peroxidase (MnP) production of Phanerochaete chrysosporium cultures were studied. In the cultures supplemented daily with 300 U/L GOx after beginning of secondary metabolite, MnP activity was increased 4 against culture medium control (The cultures that did not receive glucose and glucose oxidase) on day 15. In addition, remarkable increase in stability of MnP productivity by fungi, in cultures supplemented daily with GOx glucose (more than 32 days), as compared with control cultures (22 days) was observed. Neither H2O2 nor gluconic acid produced by GOx was responsible for improvement of peroxidase activity in nitrogen-limited cultures. The novel findings may be important in improving the yield of lignin modifying enzymes for biodegradation of lignin and a broad range of man-made aromatic compounds In the second part, decolorization crystal violet dye as a model of culture that had received glucose was studied. The results of three factors manganese peroxidase enzyme glucose oxidase activity ratio (x1), dye concentration (..2) and glucose (..3) showed that the effect of the first factor in the optimization of this process is much more than changes in dye concentration and glucose. So that by increasing the amount of factor 1 activity, color removal rate to 70 percent