NOTES PERTAINING TO PUBLICATION, DISTRIBUTION, ETC.
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Electronic
INTERNAL BIBLIOGRAPHIES/INDEXES NOTE
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Includes bibliographical references and index.
CONTENTS NOTE
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Tripartite fusion system for the selection of protein variants with increased stability in vivo /Linda Foit and James C.A. Bardwell --Determining enzyme kinetics via isothermal titration calorimetry /Neil A. Demarse ... [et al.] --GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria /Olga Paley ... [et al.] --Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties /Sarah K. Baxter ... [et al.] --TAL effector nuclease (TALEN) engineering /Ting Li and Bing Yang --In vitro evolution of enzymes /Misha V. Golynskiy ... [et al.] --Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo /Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington --Reconstructing evolutionary adaptive paths for protein engineering /Megan F. Cole ... [et al.] --Oligonucleotide recombination enabled site-specific mutagenesis in bacteria /Bryan M. Swingle --FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants /Eric R. Geertsma --Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes /Hidehiko Hirakawa and Teruyuki Nagamune --Gene synthesis by assembly of deoxyuridine-containing oligonucleotides /Romualdas Vaisvila and Jurate Bitinaite --Protein engineering : single or multiple site-directed mutagenesis /Pei-Chung Hsieh and Romualdas Vaisvila --Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination /Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish --Promiscuity-based enzyme selection for rational directed evolution experiments /Sandeep Chakraborty ... [et al.] --Rational protein sequence diversification by multi-codon scanning mutagenesis /Jia Liu and T. Ashton Cropp --Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter /Jian-Wei Liu and David L. Ollis --In vitro directed evolution of enzymes expressed by E. coli in microtiter plates /Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.