عنوان

Comparative hepatic oxidative biotransformation in domestic avian species

Number

TLpq304130312

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Comparative hepatic oxidative biotransformation in domestic avian species

General Material Designation

[Thesis]

First Statement of Responsibility

J. L. Sutherlin

Subsequent Statement of Responsibility

C. R. S. Short, David H.

Name of Publisher, Distributor, etc.

Louisiana State University and Agricultural & Mechanical College

Date of Publication, Distribution, etc.

1994

Specific Material Designation and Extent of Item

302

Dissertation or thesis details and type of degree

Ph.D.

Body granting the degree

Louisiana State University and Agricultural & Mechanical College

Text preceding or following the note

1994

Text of Note

This study characterized the oxidative biotransformation capacity of the hepatic cytochrome P-450 system of selected avian species representing the Galliformes (chicken, turkey and Japanese quail) and Anseriformes (goose, Pekin duck, Khaki Campbell duck, and Muscovy duck) orders. Two objectives were addressed; first, important biotransformation pathways were evaluated in economically major and minor food-producing avian species to provide a framework on which to base drug approval decisions in these species. Second, the potential use of the selected species as sentinel organisms utilizing cytochrome P-450 induction as a biomarker of environmental chemical exposure was evaluated. The male rat was utilized for the mammalian model and as a point of reference. Two groups of animals for each species were evaluated, a control group, which received no treatment, and a treatment group which was administered usd\betausd-naphthoflavone (usd\betausdNF). Investigations included the quantitation of microsomal protein, cytochrome P-450, NADH- and NADPH-cytochrome c reductase activity, ethoxyresorufin O-deethylase activity and benzo (a) pyrene hydroxylase activity with the development of profiles for four major metabolites. This study found that constitutive microsomal protein levels, cytochrome P-450 content, NADPH-cytochrome c reductase activities and ethoxyresorufin O-deethylase activities were similar among the species studied while NADH-cytochrome c reductase activities differed. In response to usd\betausdNF, microsomal protein levels, cytochrome P-450 content, and ethoxyresorufin O-deethylase activities were increased but no change was observed in NADPH- or NADH-cytochrome c reductase activities. Interspecies differences were observed in the response of the hepatic microsomal system to usd\betausdNF treatment. Interspecies differences were also observed in the capacity of the avian hepatic microsomal system to hydroxylate benzo (a) pyrene and in the effect of usd\betausdNF treatment upon metabolite production. 3-Hydroxybenzo (a) pyrene was the primary metabolite produced by all the species studied; interspecies and interorder differences in product distributions were observed for the 9-hydroxybenzo (a) pyrene, benzo (a) pyrene 7,8-dihydrodiol and benzo (a) pyrene 9,10-dihydrodiol metabolites.

Biological sciences

Health and environmental sciences

Toxicology

Toxicology

Veterinary services

C. R. S. Short, David H.

J. L. Sutherlin

Electronic name

p

[Thesis]

276903

a

Y