عنوان

Genetic variation in strains of Clavibacter michiganensis subsp. michiganensis and the development of bird's eye fruit lesions on tomatoes

Number

TLpq304522779

.Language of Text, Soundtrack etc

انگلیسی

Title Proper

Genetic variation in strains of Clavibacter michiganensis subsp. michiganensis and the development of bird's eye fruit lesions on tomatoes

General Material Designation

[Thesis]

First Statement of Responsibility

C. M. Medina-Mora

Name of Publisher, Distributor, etc.

Michigan State University

Date of Publication, Distribution, etc.

1999

Specific Material Designation and Extent of Item

145

Dissertation or thesis details and type of degree

M.S.

Body granting the degree

Michigan State University

Text preceding or following the note

1999

Text of Note

Bacterial canker of tomato is a major concern worldwide because disease occurrence is sporadic and may result in large yield reductions. Computer-assisted analysis of repetitive sequence-based Polymerase Chain Reaction (rep-PCR) genomic fingerprints generated with the universal primers BOX and (GTG)5 suggested genomic variation in Clavibacter michiganensis subsp. michiganensis (Cmm) is relatively limited. However, (GTG)5-PCR fingerprint patterns do differentiate strains within the previously designated BOX-PCR types (A, B, C, D, and E). Intraspecific diversity using combined (GTG)5-PCR and BOX-PCR data is as follows; usd\rm C>A\ge B>D>Eusd. These data may provide useful genetic markers to trace Cmm strains used in epidemiological studies. Fruit spotting resulting from Cmm infection is one of the most unpredictable tomato disease symptoms of bacterial canker in Michigan. Therefore, fruit spot formation was studied by spraying flowers with Cmm at various developmental stages. Maximum incidence of spotted fruit and maximum severity of fruit lesions resulted when inoculum was applied twice, a possible indication that a bacterial population threshold is required for the onset of fruit spots. Chemical control strategies could be formulated to reduce the level of unmarketable tomato fruit.

Biological sciences

Genetics

Plant pathology

C. M. Medina-Mora

Electronic name

p

[Thesis]

276903

a

Y